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A8082 B-RAF (Phospho-Ser446) Antibody
点击次数:125发布时间:2012/8/30 16:38:02
更新日期:2024/10/16 15:59:19
所 在 地:美洲
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A8082 B-RAF (Phospho-Ser446) Antibody
Reactivity | Applications | MW. | Source |
H, M | WB IHC ELISA | 84 kDa | Rabbit |
Product Name | B-RAF (Phospho-Ser446) Antibody |
Synonyms | B-RAF; B-RAF proto-oncogene serine/threonine-protein kinase; B-Raf proto-oncogene serine/threonine-protein kinase; BRAF; BRAF1; c-RMIL; kinase B-Raf; p94; RAFB1; RMIL; RMIL serine/threonine-protein kinase; v-Raf murine sarcoma viral oncogene homolog B1 |
Immunogen | The antiserum was produced against synthesized peptide derived from human B-RAF around the phosphorylation site of Ser446. |
Immunogen Region | Internal |
Clonality | Polyclonal |
Modification sites | H:S446 M:S483 |
Application dilutions | WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:10000 |
Specificity | B-RAF (Phospho-Ser446) Antibody detects endogenous levels of B-RAF only when phosphorylated at serine446. |
Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation. |
Physical Form | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Concentration | 1 mg/ml |
Storage/Stability | Store at -20℃/1 year |
NCBI Gene Symbol | BRAF1 | ||||||||
Database Links |
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Application images | Immunohistochemistry analysis of paraffin-embedded human brain, using B-RAF (Phospho-Ser446) Antibody. The picture on the right is treated with the synthesized peptide. Western blot analysis of extracts from 293 cells treated with EGF 200ng/ml 30', using B-RAF (Phospho-Ser446) Antibody. The lane on the right is treated with the synthesized peptide. |
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