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A1193 CD227/MUC1 (Phospho-Tyr1229) Antibody
点击次数:134发布时间:2012/8/31 21:48:51
更新日期:2024/10/16 15:59:19
所 在 地:美洲
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A1193 CD227/MUC1 (Phospho-Tyr1229) Antibody
Reactivity | Applications | MW. | Source |
H | WB IHC IF ELISA | 122 kDa | Rabbit |
Product Name | CD227/MUC1 (Phospho-Tyr1229) Antibody |
Synonyms | Breast carcinoma-associated antigen DF3; Carcinoma-associated mucin; CD227; EMA; Episialin; H23AG; MUC-1; mucin 1; Mucin 1 precursor; mucin 1, transmembrane; Peanut-reactive urinary mucin; PEM; PEMT; Polymorphic epithelial mucin; PUM; Tumor-associated epithelial membrane antigen; Tumor-associated mucin |
Immunogen | The antiserum was produced against synthesized peptide derived from human CD227/MUC1 around the phosphorylation site of Tyr1229. |
Immunogen Region | C-terminal |
Clonality | Polyclonal |
Modification sites | H:Y1229 |
Application dilutions | WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:1000 |
Specificity | CD227/MUC1 (Phospho-Tyr1229) Antibody detects endogenous levels of CD227/MUC1 only when phosphorylated at tyrosine1229. |
Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation. |
Physical Form | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Concentration | 1 mg/ml |
Storage/Stability | Store at -20℃/1 year |
NCBI Gene Symbol | MUC1 | ||||||||
Database Links |
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Application images | Immunofluorescence analysis of HepG2 cells, using CD227/MUC1 (Phospho-Tyr1229) Antibody. The picture on the right is treated with the synthesized peptide. Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using CD227/MUC1 (Phospho-Tyr1229) Antibody. The picture on the right is treated with the synthesized peptide. Western blot analysis of extracts from HepG2 cells treated with PMA 125ng/ml 30', using CD227/MUC1 (Phospho-Tyr1229) Antibody. The lane on the right is treated with the synthesized peptide. |
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