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A0518 MYPT1 (Phospho-Thr853) Antibody
点击次数:156发布时间:2012/9/2 10:43:09
更新日期:2024/10/16 15:59:19
所 在 地:美洲
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A0518 MYPT1 (Phospho-Thr853) Antibody
Reactivity | Applications | MW. | Source |
H, M, R | WB IHC ELISA | 115 kDa | Rabbit |
Product Name | MYPT1 (Phospho-Thr853) Antibody |
Synonyms | MBS; Myosin phosphatase target subunit 1; PPP1R12A |
Immunogen | The antiserum was produced against synthesized peptide derived from human MYPT1 around the phosphorylation site of Thr853. |
Immunogen Region | Internal |
Clonality | Polyclonal |
Modification sites | H:T853 M:T852 R:T855 |
Application dilutions | WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:1000 |
Specificity | MYPT1 (Phospho-Thr853) Antibody detects endogenous levels of MYPT1 only when phosphorylated at threonine853. |
Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation. |
Physical Form | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Concentration | 1 mg/ml |
Storage/Stability | Store at -20℃/1 year |
NCBI Gene Symbol | PPP1R12A | ||||||||
Database Links |
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Application images | Immunohistochemistry analysis of paraffin-embedded human colon carcinoma, using MYPT1 (Phospho-Thr853) Antibody. The picture on the right is treated with the synthesized peptide. Western blot analysis of extracts from NIH-3T3 cells, using MYPT1 (Phospho-Thr853) Antibody. The lane on the right is treated with the synthesized peptide. |
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