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A0021 NF-kappaB p105/p50 （Phospho-Ser927） Antibody
| Reactivity | Applications | MW. | Source |
| H, M | WB IHC IF ELISA | 105 kDa | Rabbit |
Note: The product is for research use only, not for use in diagnostic or therapeutic procedures.
| Product Name | NF-kappaB p105/p50 （Phospho-Ser927） Antibody |
| Synonyms | DNA-binding factor KBF1; EBP-1; NF-kappa-B1 p84/NF-kappa-B1 p98; NFKB1; NFkB-p50; Nuclear factor NF-kappa-B p105 subunit; nuclear factor NF-kappa-B p50 subunit |
| Immunogen | The antiserum was produced against synthesized peptide derived from human NF-kappaB p105/p50 around the phosphorylation site of Ser927. |
| Immunogen Region | C-terminal |
| Clonality | Polyclonal |
| Modification sites | H:S927 M:S930 |
| Application dilutions | WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:20000 |
| Specificity | NF-kappaB p105/p50 （Phospho-Ser927） Antibody detects endogenous levels of NF-kappaB p105/p50 only when phosphorylated at serine927. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation. |
| Physical Form | Rabbit IgG in phosphate buffered saline （without Mg2+ ａnd Ca2+）, pH 7.4, 150mM NaCl, 0.02% sodium azide ａnd 50% glycerol. |
| Concentration | 1 mg/ml |
| Storage/Stability | Store at -20℃/1 year |
| NCBI Gene Symbol | NFKB1 | ||||||||
| Database Links |
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| Application images |  Immunofluorescence analysis of HeLa cells treated with EGF 200nM 5＇, using NF-kappaB p105/p50 （Phospho-Ser927） Antibody. The picture on the right is treated with the synthesized peptide.  Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using NF-kappaB p105/p50 （Phospho-Ser927） Antibody. The picture on the right is treated with the synthesized peptide.  Western blot analysis of extracts from HeLa cells treated with LPS 100ng/ml 30＇, using NF-kappaB p105/p50 （Phospho-Ser927） Antibody. The lane on the right is treated with the synthesized peptide. |
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