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A7162 NF-kappaB p65 (Phospho-Ser529) Antibody
点击次数:121发布时间:2012/9/2 14:08:56
更新日期:2024/10/16 15:59:19
所 在 地:美洲
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A7162 NF-kappaB p65 (Phospho-Ser529) Antibody
Reactivity | Applications | MW. | Source |
H, M | WB IHC ELISA | 60 kDa | Rabbit |
Product Name | NF-kappaB p65 (Phospho-Ser529) Antibody |
Synonyms | NFKB3; nuclear factor NF-kappa-B p65 subunit; p65; RELA; TF65; transcription factor p65 |
Immunogen | The antiserum was produced against synthesized peptide derived from human NF-kappaB p65 around the phosphorylation site of Ser529. |
Immunogen Region | C-terminal |
Clonality | Polyclonal |
Modification sites | H:S529 M:S528 |
Application dilutions | WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:10000 |
Specificity | NF-kappaB p65 (Phospho-Ser529) Antibody detects endogenous levels of NF-kappaB p65 only when phosphorylated at serine529. |
Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation. |
Physical Form | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Concentration | 1 mg/ml |
Storage/Stability | Store at -20℃/1 year |
NCBI Gene Symbol | RELA | ||||||||
Database Links |
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Application images | Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using NF-kappaB p65 (Phospho-Ser529) Antibody. The picture on the right is treated with the synthesized peptide. Western blot analysis of extracts from HeLa and Jurkat cells, using NF-kappaB p65 (Phospho-Ser529) Antibody. The lane on the right is treated with the synthesized peptide. |
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