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A7173 NF-kappaB p65 (Phospho-Thr435) Antibody
点击次数：137发布时间：2012/9/2 14:11:30
更新日期：2024/10/16 15:59:19
所 在 地：美洲
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A7173 NF-kappaB p65 （Phospho-Thr435） Antibody
| Reactivity | Applications | MW. | Source |
| H, M, R | WB IHC IP ELISA | 60 kDa | Rabbit |
Note: The product is for research use only, not for use in diagnostic or therapeutic procedures.
| Product Name | NF-kappaB p65 （Phospho-Thr435） Antibody |
| Synonyms | NFKB3; nuclear factor NF-kappa-B p65 subunit; p65; RELA; TF65; transcription factor p65 |
| Immunogen | The antiserum was produced against synthesized peptide derived from human NF-kappaB p65 around the phosphorylation site of Thr435. |
| Immunogen Region | C-terminal |
| Clonality | Polyclonal |
| Modification sites | H:T435 M:T433 R:T434 |
| Application dilutions | WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:20000 |
| Specificity | NF-kappaB p65 （Phospho-Thr435） Antibody detects endogenous levels of NF-kappaB p65 only when phosphorylated at threonine435. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation. |
| Physical Form | Rabbit IgG in phosphate buffered saline （without Mg2+ ａnd Ca2+）, pH 7.4, 150mM NaCl, 0.02% sodium azide ａnd 50% glycerol. |
| Concentration | 1 mg/ml |
| Storage/Stability | Store at -20℃/1 year |
| NCBI Gene Symbol | RELA | ||||||||
| Database Links |
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| Application images |  Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using NF-kappaB p65 （Phospho-Thr435） Antibody. The picture on the right is treated with the synthesized peptide.  Western blot analysis of extracts from COS7 cells treated with TNF-alpha, using NF-kappaB p65 （Phospho-Thr435） Antibody. The lane on the right is treated with the synthesized peptide. |
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