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A0694 NPM (Phospho-Thr199) Antibody
点击次数:142发布时间:2012/9/2 15:14:53
更新日期:2024/10/16 15:59:31
所 在 地:美洲
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A0694 NPM (Phospho-Thr199) Antibody
Reactivity | Applications | MW. | Source |
H, M, R | WB IHC IF ELISA | 32 kDa | Rabbit |
Product Name | NPM (Phospho-Thr199) Antibody |
Synonyms | B23.1; NPM isoform 1; NPM1; Nucleolar phosphoprotein B23; Nucleolar protein NO38; nucleophosmin; nucleophosmin (nucleolar phosphoprotein B23, numatrin); Numatrin |
Immunogen | The antiserum was produced against synthesized peptide derived from human NPM around the phosphorylation site of Thr199. |
Immunogen Region | Internal |
Clonality | Polyclonal |
Modification sites | H:T199 M:T198 R:T198 |
Application dilutions | WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:60000 |
Specificity | NPM (Phospho-Thr199) Antibody detects endogenous levels of NPM only when phosphorylated at threonine199. |
Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation. |
Physical Form | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Concentration | 1 mg/ml |
Storage/Stability | Store at -20℃/1 year |
NCBI Gene Symbol | NPM1 | ||||||||
Database Links |
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Application images | Immunofluorescence analysis of HeLa cells treated with EGF 200nM 5', using NPM (Phospho-Thr199) Antibody. The picture on the right is treated with the synthesized peptide. Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using NPM (Phospho-Thr199) Antibody. The picture on the right is treated with the synthesized peptide. Western blot analysis of extracts from HepG2 cells, using NPM (Phospho-Thr199) Antibody. The lane on the right is treated with the synthesized peptide. |
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