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A0533 p70 S6 Kinase (Phospho-Thr389) Antibody
Reactivity | Applications | MW. | Source |
H, M, R | WB IHC IF ELISA | 59 kDa | Rabbit |
Product Name | p70 S6 Kinase (Phospho-Thr389) Antibody |
Synonyms | Ribosomal protein S6 kinase I; S6K; S6K1; 70 kDa ribosomal protein S6 kinase 1; p70 S6 kinase alpha; p70(S6K)-alpha; p70-S6K; P70S6K; p70-alpha |
Immunogen | The antiserum was produced against synthesized peptide derived from human p70 S6 Kinase around the phosphorylation site of Thr389. |
Immunogen Region | Internal |
Clonality | Polyclonal |
Modification sites | H:T412 M:T412 R:T412 |
Application dilutions | WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:1000 |
Specificity | p70 S6 Kinase (Phospho-Thr389) Antibody detects endogenous levels of p70 S6 Kinase only when phosphorylated at threonine389. |
Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation. |
Physical Form | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Concentration | 1 mg/ml |
Storage/Stability | Store at -20℃/1 year |
NCBI Gene Symbol | RPS6KB1 | ||||||||
Database Links |
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Application images | Immunofluorescence analysis of HuvEc cells, using p70 S6 Kinase (Phospho-Thr389) Antibody. The picture on the right is treated with the synthesized peptide. Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using p70 S6 Kinase (Phospho-Thr389) Antibody. The picture on the right is treated with the synthesized peptide. Western blot analysis of extracts from Jurkat cells treated with Insulin 0.01U/ml 15', using p70 S6 Kinase (Phospho-Thr389) Antibody. The lane on the right is treated with the synthesized peptide. |