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A0539 PAK1 (Phospho-Thr212) Antibody
点击次数:102发布时间:2012/9/4 10:26:03
更新日期:2024/10/16 15:59:31
所 在 地:美洲
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A0539 PAK1 (Phospho-Thr212) Antibody
Reactivity | Applications | MW. | Source |
H, M, R | WB IHC ELISA | 60 kDa | Rabbit |
Product Name | PAK1 (Phospho-Thr212) Antibody |
Synonyms | Serine/threonine-protein kinase PAK 1; p21-activated kinase 1; PAK-1; p65-PAK; Alpha-PAK; PAK1 |
Immunogen | The antiserum was produced against synthesized peptide derived from human PAK1 around the phosphorylation site of Thr212. |
Immunogen Region | Internal |
Clonality | Polyclonal |
Modification sites | H:T212 M:T212 R:T211 |
Application dilutions | WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:1000 |
Specificity | PAK1 (Phospho-Thr212) Antibody detects endogenous levels of PAK1 only when phosphorylated at threonine212. |
Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation. |
Physical Form | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Concentration | 1 mg/ml |
Storage/Stability | Store at -20℃/1 year |
NCBI Gene Symbol | PAK1 | ||||||||
Database Links |
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Application images | Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using PAK1 (Phospho-Thr212) Antibody. The picture on the right is treated with the synthesized peptide. Western blot analysis of extracts from 293 cells treated with etoposide 25uM 1h, using PAK1 (Phospho-Thr212) Antibody. The lane on the right is treated with the synthesized peptide. |
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