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A0803 PKC-pan (Phospho-Thr497) Antibody
点击次数:197发布时间:2012/9/4 16:17:12
更新日期:2024/10/16 15:59:31
所 在 地:美洲
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A0803 PKC-pan (Phospho-Thr497) Antibody
Reactivity | Applications | MW. | Source |
H, M, R | WB IHC IF ELISA | 76 kDa | Rabbit |
Product Name | PKC-pan (Phospho-Thr497) Antibody |
Synonyms | KPCA; PKC III; PKC-A; PKC-III; PKC-alpha; PRKACA; PRKCA; Protein kinase C, alpha type; kinase PKC-alpha PKC-delta; PRKCD; kinase PKC-delta; nPKC-delta PKC-epsilon; PKCEA; PRKCE, epsilon type; kinase PKC-epsilon; nPKC-epsilon |
Immunogen | The antiserum was produced against synthesized peptide derived from human PKC-pan around the phosphorylation site of Thr497. |
Immunogen Region | C-terminal |
Clonality | Polyclonal |
Modification sites | H:T497/500/507/566/514/512/538/410 M:T497/500/505/566/514/513/538/410 R:T497/500/505/566/514/513/538/410 |
Application dilutions | WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:40000 |
Specificity | PKC-pan (Phospho-Thr497) Antibody detects endogenous levels of PKC-pan only when phosphorylated at threonine497. |
Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation. |
Physical Form | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Concentration | 1 mg/ml |
Storage/Stability | Store at -20℃/1 year |
NCBI Gene Symbol | PRKCA | ||||||||
Database Links |
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Application images | Immunofluorescence analysis of HepG2 cells, using PKC-pan (Phospho-Thr497) Antibody. The picture on the right is treated with the synthesized peptide. Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using PKC-pan (Phospho-Thr497) Antibody. The picture on the right is treated with the synthesized peptide. Western blot analysis of extracts from NIH-3T3 cells treated with PMA 250ng/ml 15', using PKC-pan (Phospho-Thr497) Antibody. The lane on the right is treated with the synthesized peptide. |
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