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A0566 C-RAF (Phospho-Ser621) Antibody
点击次数：147发布时间：2012/9/4 18:34:56
更新日期：2024/10/16 15:59:31
所 在 地：美洲
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A0566 C-RAF （Phospho-Ser621） Antibody
| Reactivity | Applications | MW. | Source |
| H, M, R | WB IHC IF ELISA | 73 kDa | Rabbit |
Note: The product is for research use only, not for use in diagnostic or therapeutic procedures.
| Product Name | C-RAF （Phospho-Ser621） Antibody |
| Synonyms | C-RAF; C-Raf; CRAF; kinase Raf1; Protein kinase raf 1; RAF proto-oncogene serine/threonine-protein kinase; Raf proto-oncogene serine/threonine-protein kinase; RAF-1; Raf-1; RAF1; Similar to murine leukemia viral （V-raf-1） oncogene homolog 1 |
| Immunogen | The antiserum was produced against synthesized peptide derived from human C-RAF around the phosphorylation site of Ser621. |
| Immunogen Region | C-terminal |
| Clonality | Polyclonal |
| Modification sites | H:Y621 M:Y621 R:Y621 |
| Application dilutions | WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:5000 |
| Specificity | C-RAF （Phospho-Ser621） Antibody detects endogenous levels of C-RAF only when phosphorylated at serine621. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation. |
| Physical Form | Rabbit IgG in phosphate buffered saline （without Mg2+ ａnd Ca2+）, pH 7.4, 150mM NaCl, 0.02% sodium azide ａnd 50% glycerol. |
| Concentration | 1 mg/ml |
| Storage/Stability | Store at -20℃/1 year |
| NCBI Gene Symbol | RAF1 | ||||||||
| Database Links |
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| Application images |  Immunofluorescence analysis of HeLa cells, using C-RAF （Phospho-Ser621） Antibody. The picture on the right is treated with the synthesized peptide.  Immunohistochemistry analysis of paraffin-embedded human lung carcinoma, using C-RAF （Phospho-Ser621） Antibody. The picture on the right is treated with the synthesized peptide.  Western blot analysis of extracts from HeLa cells treated with UV 5＇, using C-RAF （Phospho-Ser621） Antibody. The lane on the right is treated with the synthesized peptide. |
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