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A0066 CrkII (Phospho-Tyr221) Antibody
点击次数：142发布时间：2012/9/4 20:28:10
更新日期：2024/10/16 15:59:43
所 在 地：美洲
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A0066 CrkII （Phospho-Tyr221） Antibody
| Reactivity | Applications | MW. | Source |
| H, M, R | WB IF ELISA | 33 kDa | Rabbit |
Note: The product is for research use only, not for use in diagnostic or therapeutic procedures.
| Product Name | CrkII （Phospho-Tyr221） Antibody |
| Synonyms | Adapter molecule crk; CRKO; Crk-II; P38; Proto-oncogene C-crk |
| Immunogen | The antiserum was produced against synthesized peptide derived from human CrkII around the phosphorylation site of Tyr221. |
| Immunogen Region | Internal |
| Clonality | Polyclonal |
| Modification sites | H:Y221 M:Y221 R:Y221 |
| Application dilutions | WB: 1:500~1:1000 IF: 1:100~1:500 ELISA: 1:40000 |
| Specificity | CrkII （Phospho-Tyr221） Antibody detects endogenous levels of CrkII only when phosphorylated at tyrosine221. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation. |
| Physical Form | Rabbit IgG in phosphate buffered saline （without Mg2+ ａnd Ca2+）, pH 7.4, 150mM NaCl, 0.02% sodium azide ａnd 50% glycerol. |
| Concentration | 1 mg/ml |
| Storage/Stability | Store at -20℃/1 year |
| NCBI Gene Symbol | CRK | ||||||||
| Database Links |
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| Application images |  Immunofluorescence analysis of HuvEc cells, using CrkII （Phospho-Tyr221） Antibody. The picture on the right is treated with the synthesized peptide.  Western blot analysis of extracts from COS7 cells, using CrkII （Phospho-Tyr221） Antibody. The lane on the right is treated with the synthesized peptide. |
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