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B0082 POLR2A (Ab-1619) Antibody
Reactivity | Applications | MW. | Source |
H, M, R | WB IHC IF ELISA | 217 kDa | Rabbit |
Note: The product is for research use only, not for use in diagnostic or therapeutic procedures.
Product Name | POLR2A (Ab-1619) Antibody |
Synonyms | DNA-directed RNA polymerase II largest subunit; RNA pol II largest subunit; RNA polymerase II 220 kd subunit; RPB1 |
Immunogen | The antiserum was produced against synthesized peptide derived from human POLR2A. |
Immunogen Region | C-terminal |
Clonality | Polyclonal |
Application dilutions | WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:10000 |
Specificity | POLR2A (Ab-1619) Antibody detects endogenous levels of total POLR2A protein. |
Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
Physical Form | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Concentration | 1 mg/ml |
Storage/Stability | Store at -20℃/1 year |
NCBI Gene Symbol | RPB1 |
Database Links | |
Application images | Immunofluorescence analysis of HeLa cells, using POLR2A (Ab-1619) Antibody. The picture on the right is treated with the synthesized peptide. Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using POLR2A (Ab-1619) Antibody. The picture on the right is treated with the synthesized peptide. Western blot analysis of extracts from COS7 cells, treated with EGF 200ng/ml 30', using POLR2A (Ab-1619) Antibody. The lane on the right is treated with the synthesized peptide. |