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A7056 DARPP-32 (Phospho-Thr34) Antibody
点击次数：167发布时间：2012/9/5 19:13:49
更新日期：2024/10/16 15:59:43
所 在 地：美洲
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A7056 DARPP-32 （Phospho-Thr34） Antibody
| Reactivity | Applications | MW. | Source |
| H, M, R | WB IHC IF ELISA | 22 kDa | Rabbit |
Note: The product is for research use only, not for use in diagnostic or therapeutic procedures.
| Product Name | DARPP-32 （Phospho-Thr34） Antibody |
| Synonyms | Dopamine- ａnd cAMP-regulated neuronal phosphoprotein; IPPD; Neuronal phosphoprotein DARPP-32; PPP1R1B |
| Immunogen | The antiserum was produced against synthesized peptide derived from human DARPP-32 around the phosphorylation site of Thr34. |
| Immunogen Region | N-terminal |
| Clonality | Polyclonal |
| Modification sites | H:T34 M:T34 R:T34 |
| Application dilutions | WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:10000 |
| Specificity | DARPP-32 （Phospho-Thr34） Antibody detects endogenous levels of DARPP-32 only when phosphorylated at threonine34. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation. |
| Physical Form | Rabbit IgG in phosphate buffered saline （without Mg2+ ａnd Ca2+）, pH 7.4, 150mM NaCl, 0.02% sodium azide ａnd 50% glycerol. |
| Concentration | 1 mg/ml |
| Storage/Stability | Store at -20℃/1 year |
| NCBI Gene Symbol | PPP1R1B | ||||||||
| Database Links |
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| Application images |  Immunofluorescence analysis of HepG2 cells, using DARPP-32 （Phospho-Thr34） Antibody. The picture on the right is treated with the synthesized peptide.  Immunohistochemistry analysis of paraffin-embedded human brain, using DARPP-32 （Phospho-Thr34） Antibody. The picture on the right is treated with the synthesized peptide.  Western blot analysis of extracts from NIH-3T3 cells treated with PMA 125ng/ml 30＇, using DARPP-32 （Phospho-Thr34） Antibody. The lane on the right is treated with the synthesized peptide. |
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