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A0007 DARPP-32 (Phospho-Thr75) Antibody
点击次数:125发布时间:2012/9/5 19:15:20
更新日期:2024/10/16 15:59:43
所 在 地:美洲
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A0007 DARPP-32 (Phospho-Thr75) Antibody
Reactivity | Applications | MW. | Source |
H, M, R | WB IHC ELISA | 22 kDa | Rabbit |
Product Name | DARPP-32 (Phospho-Thr75) Antibody |
Synonyms | Dopamine- and cAMP-regulated neuronal phosphoprotein; IPPD; Neuronal phosphoprotein DARPP-32; PPP1R1B |
Immunogen | The antiserum was produced against synthesized peptide derived from human DARPP-32 around the phosphorylation site of Thr75. |
Immunogen Region | Internal |
Clonality | Polyclonal |
Modification sites | H:T75 M:T75 R:T75 |
Application dilutions | WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:40000 |
Specificity | DARPP-32 (Phospho-Thr75) Antibody detects endogenous levels of DARPP-32 only when phosphorylated at threonine75. |
Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation. |
Physical Form | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Concentration | 1 mg/ml |
Storage/Stability | Store at -20℃/1 year |
NCBI Gene Symbol | PPP1R1B | ||||||||
Database Links |
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Application images | Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using DARPP-32 (Phospho-Thr75) Antibody. The picture on the right is treated with the synthesized peptide. Western blot analysis of extracts from COS7 cells treated with Forskolin 40nM 30', using DARPP-32 (Phospho-Thr75) Antibody. The lane on the right is treated with the synthesized peptide. |
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