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A0423 E2F1 (Phospho-Thr433) Antibody
点击次数：193发布时间：2012/9/6 10:17:06
更新日期：2024/10/16 15:59:43
所 在 地：美洲
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A0423 E2F1 （Phospho-Thr433） Antibody
| Reactivity | Applications | MW. | Source |
| H, M | WB ELISA | 46 kDa | Rabbit |
Note: The product is for research use only, not for use in diagnostic or therapeutic procedures.
| Product Name | E2F1 （Phospho-Thr433） Antibody |
| Synonyms | E2F-1; PBR3; PRB-binding protein E2F-1; RBAP-1; RBBP-3; RBBP3; Retinoblastoma binding protein 3; Retinoblastoma-associated protein 1; Transcription factor E2F1 |
| Immunogen | The antiserum was produced against synthesized peptide derived from human E2F1 around the phosphorylation site of Thr433. |
| Immunogen Region | C-terminal |
| Clonality | Polyclonal |
| Modification sites | H:T433 M:T426 |
| Application dilutions | WB: 1:500~1:1000 ELISA: 1:5000 |
| Specificity | E2F1 （Phospho-Thr433） Antibody detects endogenous levels of E2F1 only when phosphorylated at threonine433. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation. |
| Physical Form | Rabbit IgG in phosphate buffered saline （without Mg2+ ａnd Ca2+）, pH 7.4, 150mM NaCl, 0.02% sodium azide ａnd 50% glycerol. |
| Concentration | 1 mg/ml |
| Storage/Stability | Store at -20℃/1 year |
| NCBI Gene Symbol | E2F1 | ||||||||
| Database Links |
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| Application images |  Western blot analysis of extracts from HeLa cells treated with Etoposide 25uM 24h, using E2F1 （Phospho-Thr433） Antibody. The lane on the right is treated with the synthesized peptide. |
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