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A0070 eEF2 (Phospho-Thr56) Antibody
点击次数:194发布时间:2012/9/6 10:53:55
更新日期:2024/10/16 15:59:43
所 在 地:美洲
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A0070 eEF2 (Phospho-Thr56) Antibody
Reactivity | Applications | MW. | Source |
H, M, R | WB IHC IF ELISA | 95 kDa | Rabbit |
Product Name | eEF2 (Phospho-Thr56) Antibody |
Synonyms | EEF2; EF-2; EF2; Elongation factor 2 |
Immunogen | The antiserum was produced against synthesized peptide derived from human eEF2 around the phosphorylation site of Thr56. |
Immunogen Region | N-terminal |
Clonality | Polyclonal |
Modification sites | H:T56 M:T56 R:T56 |
Application dilutions | WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:10000 |
Specificity | eEF2 (Phospho-Thr56) Antibody detects endogenous levels of eEF2 only when phosphorylated at threonine56. |
Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation. |
Physical Form | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Concentration | 1 mg/ml |
Storage/Stability | Store at -20℃/1 year |
NCBI Gene Symbol | EEF2 | ||||||||
Database Links |
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Application images | Immunofluorescence analysis of HuvEc cells, using eEF2 (Phospho-Thr56) Antibody. The picture on the right is treated with the synthesized peptide. Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using eEF2 (Phospho-Thr56) Antibody. The picture on the right is treated with the synthesized peptide. Western blot analysis of extracts from NIH-3T3 cells treated with Serum 10% 30', using eEF2 (Phospho-Thr56) Antibody. The lane on the right is treated with the synthesized peptide. |
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