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A0475 EGFR (Phospho-Tyr1016) Antibody
点击次数：168发布时间：2012/9/6 12:32:27
更新日期：2024/10/16 15:59:43
所 在 地：美洲
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A0475 EGFR （Phospho-Tyr1016） Antibody
| Reactivity | Applications | MW. | Source |
| H, M, R | WB IHC IF ELISA | 134 kDa | Rabbit |
Note: The product is for research use only, not for use in diagnostic or therapeutic procedures.
| Product Name | EGFR （Phospho-Tyr1016） Antibody |
| Synonyms | Epidermal growth factor receptor; Proto-oncogene c-ErbB-1; Receptor tyrosine-protein kinase erbB-1 ; EGFR ; ERBB1 |
| Immunogen | The antiserum was produced against synthesized peptide derived from human EGFR around the phosphorylation site of Tyr1016. |
| Immunogen Region | C-terminal |
| Clonality | Polyclonal |
| Modification sites | H:Y1016 M:Y1018 R:Y1017 |
| Application dilutions | WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:1000 |
| Specificity | EGFR （Phospho-Tyr1016） Antibody detects endogenous levels of EGFR only when phosphorylated at tyrosine1016. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation. |
| Physical Form | Rabbit IgG in phosphate buffered saline （without Mg2+ ａnd Ca2+）, pH 7.4, 150mM NaCl, 0.02% sodium azide ａnd 50% glycerol. |
| Concentration | 1 mg/ml |
| Storage/Stability | Store at -20℃/1 year |
| NCBI Gene Symbol | EGFR | ||||||||
| Database Links |
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| Application images |  Immunofluorescence analysis of HeLa cells, using EGFR （Phospho-Tyr1016） Antibody. The picture on the right is treated with the synthesized peptide.  Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using EGFR （Phospho-Tyr1016） Antibody. The picture on the right is treated with the synthesized peptide.  Western blot analysis of extracts from HuvEc cells treated with Serum 20% 30＇, using EGFR （Phospho-Tyr1016） Antibody. The lane on the right is treated with the synthesized peptide. |
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