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A7064 EGFR (Phospho-Tyr1197) Antibody
点击次数:343发布时间:2012/9/6 12:40:25
更新日期:2024/10/16 15:59:43
所 在 地:美洲
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A7064 EGFR (Phospho-Tyr1197) Antibody
Reactivity | Applications | MW. | Source |
H, M, R | WB IHC ELISA | 134 kDa | Rabbit |
Product Name | EGFR (Phospho-Tyr1197) Antibody |
Synonyms | Epidermal growth factor receptor; Proto-oncogene c-ErbB-1; Receptor tyrosine-protein kinase erbB-1 ; EGFR ; ERBB1 |
Immunogen | The antiserum was produced against synthesized peptide derived from human EGFR around the phosphorylation site of Tyr1197. |
Immunogen Region | C-terminal |
Clonality | Polyclonal |
Modification sites | H:Y1197 M:Y1197 R:Y1196 |
Application dilutions | WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:10000 |
Specificity | EGFR (Phospho-Tyr1197) Antibody detects endogenous levels of EGFR only when phosphorylated at tyrosine1197. |
Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation. |
Physical Form | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Concentration | 1 mg/ml |
Storage/Stability | Store at -20℃/1 year |
NCBI Gene Symbol | EGFR | ||||||||
Database Links |
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Application images | Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using EGFR (Phospho-Tyr1197) Antibody. The picture on the right is treated with the synthesized peptide. Western blot analysis of extracts from A431 cells treated with EGF 40 muM 10', using EGFR (Phospho-Tyr1197) Antibody. The lane on the left is treated with the synthesized peptide. |
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