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A0641 eIF4B (Phospho-Ser422) Antibody
点击次数:89发布时间:2012/9/6 15:31:14
更新日期:2024/10/16 15:59:43
所 在 地:美洲
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A0641 eIF4B (Phospho-Ser422) Antibody
Reactivity | Applications | MW. | Source |
H, M | WB IHC IF ELISA | 69 kDa | Rabbit |
Product Name | eIF4B (Phospho-Ser422) Antibody |
Synonyms | Eukaryotic translation initiation factor 4B; IF4B; eIF-4B |
Immunogen | The antiserum was produced against synthesized peptide derived from human eIF4B around the phosphorylation site of Ser422. |
Immunogen Region | Internal |
Clonality | Polyclonal |
Modification sites | H:S422 M:S422 |
Application dilutions | WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:40000 |
Specificity | eIF4B (Phospho-Ser422) Antibody detects endogenous levels of eIF4B only when phosphorylated at serine422. |
Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation. |
Physical Form | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Concentration | 1 mg/ml |
Storage/Stability | Store at -20℃/1 year |
NCBI Gene Symbol | EIF4B | ||||||||
Database Links |
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Application images | Immunofluorescence analysis of NIH-3T3 cells, using eIF4B (Phospho-Ser422) Antibody. The picture on the right is treated with the synthesized peptide. Immunohistochemistry analysis of paraffin-embedded human colon carcinoma, using eIF4B (Phospho-Ser422) Antibody. The picture on the right is treated with the synthesized peptide. Western blot analysis of extracts from NIH-3T3 cells, using eIF4B (Phospho-Ser422) Antibody. The lane on the right is treated with the synthesized peptide. |
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