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A7067 eIF4E (Phospho-Ser209) Antibody
点击次数:113发布时间:2012/9/6 15:37:27
更新日期:2024/10/16 15:59:43
所 在 地:美洲
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A7067 eIF4E (Phospho-Ser209) Antibody
Reactivity | Applications | MW. | Source |
H, M, R | WB IHC ELISA | 25 kDa | Rabbit |
Product Name | eIF4E (Phospho-Ser209) Antibody |
Synonyms | Eukaryotic translation initiation factor 4E; eIF-4E; eIF4E; mRNA cap-binding protein; eIF-4F 25 kDa subunit; EIF4E; EIF4EL1; EIF4F |
Immunogen | The antiserum was produced against synthesized peptide derived from human eIF4E around the phosphorylation site of Ser209. |
Immunogen Region | C-terminal |
Clonality | Polyclonal |
Modification sites | H:S209 M:S209 R:S209 |
Application dilutions | WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:20000 |
Specificity | eIF4E (Phospho-Ser209) Antibody detects endogenous levels of eIF4E only when phosphorylated at serine209. |
Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation. |
Physical Form | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Concentration | 1 mg/ml |
Storage/Stability | Store at -20℃/1 year |
NCBI Gene Symbol | EIF4E | ||||||||
Database Links |
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Application images | Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using eIF4E (Phospho-Ser209) Antibody. The picture on the right is treated with the synthesized peptide. Western blot analysis of extracts from 293 cells treated with Anisomycin 25ug/ml 30', using eIF4E (Phospho-Ser209) Antibody. The lane on the right is treated with the synthesized peptide. |
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