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A1003 Smad3 (Phospho-Thr179) Antibody
点击次数:106发布时间:2012/9/7 9:52:55
更新日期:2024/10/16 15:59:55
所 在 地:美洲
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A1003 Smad3 (Phospho-Thr179) Antibody
Reactivity | Applications | MW. | Source |
H, M, R | WB IHC ELISA | 48 kDa | Rabbit |
Product Name | Smad3 (Phospho-Thr179) Antibody |
Synonyms | JV15-2; MAD-3; MADH3; Mad3; Mothers against DPP homolog 3; Mothers against decapentaplegic homolog 3; SMAD 3; Smad 3 |
Immunogen | The antiserum was produced against synthesized peptide derived from human Smad3 around the phosphorylation site of Thr179. |
Immunogen Region | Internal |
Clonality | Polyclonal |
Modification sites | H:T179 M:T179 R:T179 |
Application dilutions | WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:1000 |
Specificity | Smad3 (Phospho-Thr179) Antibody detects endogenous levels of Smad3 only when phosphorylated at threonine179. |
Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation. |
Physical Form | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Concentration | 1 mg/ml |
Storage/Stability | Store at -20℃/1 year |
NCBI Gene Symbol | SMAD3 | ||||||||
Database Links |
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Application images | Immunohistochemistry analysis of paraffin-embedded human heart, using Smad3 (Phospho-Thr179) Antibody. The picture on the right is treated with the synthesized peptide. Western blot analysis of extracts from HeLa cells treated with TNF 20ng/ml 2', using Smad3 (Phospho-Thr179) Antibody. The lane on the right is treated with the synthesized peptide. |
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