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A7253 VEGFR2 (Phospho-Tyr1214) Antibody

点击次数:95发布时间:2012/9/8 10:45:50

A7253  VEGFR2 (Phospho-Tyr1214) Antibody

更新日期:2024/10/16 16:00:07

所 在 地:美洲

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A7253  VEGFR2 (Phospho-Tyr1214) Antibody

Reactivity Applications MW. Source
H, M, R WB IHC ELISA 151 kDa Rabbit

Note: The product is for research use only, not for use in diagnostic or therapeutic procedures.

Product Name VEGFR2 (Phospho-Tyr1214) Antibody
Synonyms FLK1; KDR; kinase insert domain receptor; protein-tyrosine kinase receptor Flk-1; vascular endothelial growth factor receptor 2; VGFR2; VGR2
Immunogen The antiserum was produced against synthesized peptide derived from human VEGFR2 around the phosphorylation site of Tyr1214.
Immunogen Region C-terminal
Clonality Polyclonal
Modification sites H:Y1214 M:Y1212 R:Y1210
Application dilutions WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:10000
Specificity VEGFR2 (Phospho-Tyr1214) Antibody detects endogenous levels of VEGFR2 only when phosphorylated at tyrosine1214.
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation.
Physical Form Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration 1 mg/ml
Storage/Stability Store at -20℃/1 year

 

NCBI Gene Symbol KDR
Database Links
Application images
VEGFR2 (Phospho-Tyr1214) Antibody for Immunohistochemistry

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using VEGFR2 (Phospho-Tyr1214) Antibody. The picture on the right is treated with the synthesized peptide.

VEGFR2 (Phospho-Tyr1214) Antibody for Western Blot

Western blot analysis of extracts from HepG2 cells treated with Na3VO4 0.3nM 40', using VEGFR2 (Phospho-Tyr1214) Antibody. The lane on the right is treated with the synthesized peptide.

 

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