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A0757 YAP (Phospho-Ser127) Antibody
点击次数:115发布时间:2012/9/8 12:21:31
更新日期:2024/10/16 16:00:07
所 在 地:美洲
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A0757 YAP (Phospho-Ser127) Antibody
Reactivity | Applications | MW. | Source |
H, M, R | WB IHC ELISA | 48 kDa | Rabbit |
Product Name | YAP (Phospho-Ser127) Antibody |
Synonyms | 65 kDa Yes-associated protein; YAP1; YAP65 |
Immunogen | The antiserum was produced against synthesized peptide derived from human YAP around the phosphorylation site of Ser127. |
Immunogen Region | Internal |
Clonality | Polyclonal |
Modification sites | H:S127 M:S112 R:S112 |
Application dilutions | WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:40000 |
Specificity | YAP (Phospho-Ser127) Antibody detects endogenous levels of YAP only when phosphorylated at serine127. |
Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation. |
Physical Form | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Concentration | 1 mg/ml |
Storage/Stability | Store at -20℃/1 year |
NCBI Gene Symbol | YAP1 | ||||||||
Database Links |
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Application images | Immunohistochemistry analysis of paraffin-embedded human heart , using YAP (Phospho-Ser127) Antibody. The picture on the right is treated with the synthesized peptide. Western blot analysis of extracts from COS7 cells treated with HU 2nM 24h, using YAP (Phospho-Ser127) Antibody. The lane on the right is treated with the synthesized peptide. |