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D0005 Histone H2B (Acetyl-Lys15) Antibody
点击次数：149发布时间：2012/9/9 14:34:08
更新日期：2024/10/16 16:00:07
所 在 地：美洲
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| Reactivity | Applications | MW. | Source |
| H, M | WB IHC IF ELISA | 13 kDa | Rabbit |
Note: The product is for research use only, not for use in diagnostic or therapeutic procedures.
| Product Name | Histone H2B （Acetyl-Lys15） Antibody |
| Synonyms | Histone cluster 1; H2bb,H2B.1; H2B/f; H2BFF; H2B histone family, member F,histone 1; H2bb |
| Immunogen | The antiserum was produced against synthesized peptide derived from human Histone H2B around the acetylated site of Lys15. |
| Immunogen Region | N-terminal |
| Clonality | Polyclonal |
| Modification sites | H:K15 M:K15 |
| Application dilutions | WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:1000 |
| Specificity | Histone H2B （Acetyl-Lys15） Antibody detects endogenous levels of total Histone H2B protein only when acetylated at lysine15. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific acetylated peptide. The antibody against non-acetylated peptide was removed by chromatography using non-acetylated peptide corresponding to the acetylation site. |
| Physical Form | Rabbit IgG in phosphate buffered saline （without Mg2+ ａnd Ca2+）, pH 7.4, 150mM NaCl, 0.02% sodium azide ａnd 50% glycerol. |
| Concentration | 1 mg/ml |
| Storage/Stability | Store at -20℃/1 year |
| NCBI Gene Symbol | H2BFS | ||||||||
| Database Links |
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| Application images |  Immunofluorescence analysis of HeLa cells, using Histone H2B （Acetyl-Lys15） Antibody. The picture on the right is treated with the synthesized peptide.  Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using Histone H2B （Acetyl-Lys15） Antibody. The picture on the right is treated with the synthesized peptide.  Western blot analysis of extracts from Raw264.7 cells, treated with TSA 400nM 24h, using Histone H2B （Acetyl-Lys15） Antibody. The lane on the right is treated with the synthesized peptide. |
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