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D0008 Histone H3 (Acetyl-Lys18) Antibody
点击次数：183发布时间：2012/9/9 16:44:01
更新日期：2024/10/16 16:00:07
所 在 地：美洲
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| Reactivity | Applications | MW. | Source |
| H, M, R | WB IHC IF ELISA | 15 kDa | Rabbit |
Note: The product is for research use only, not for use in diagnostic or therapeutic procedures.
| Product Name | Histone H3 （Acetyl-Lys18） Antibody |
| Synonyms | H3/a; H3/c; H3/d; H3/f; H3/h; H3/i; H3/j; H3/k; H3/l; H31; HIST1H3A; histone H3.1 |
| Immunogen | The antiserum was produced against synthesized peptide derived from human Histone H3 around the acetylated site of Lys18. |
| Immunogen Region | N-terminal |
| Clonality | Polyclonal |
| Modification sites | H:K18 M:K18 R:K18 |
| Application dilutions | WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:5000 |
| Specificity | Histone H3 （Acetyl-Lys18） Antibody detects endogenous levels of total Histone H3 protein only when acetylated at lysine18. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific acetylated peptide. The antibody against non-acetylated peptide was removed by chromatography using non-acetylated peptide corresponding to the acetylation site. |
| Physical Form | Rabbit IgG in phosphate buffered saline （without Mg2+ ａnd Ca2+）, pH 7.4, 150mM NaCl, 0.02% sodium azide ａnd 50% glycerol. |
| Concentration | 1 mg/ml |
| Storage/Stability | Store at -20℃/1 year |
| NCBI Gene Symbol | HIST1H3A / HIST1H3B / HIST1H3C / HIST1H3D / HIST1H3E / HIST1H3F / HIST1H3G / HIST1H3H / HIST1H3I / HIST1H3J / HIST2H3A / HIST2H3C / HIST2H3D / H3F3A / H3F3B | ||||||||
| Database Links |
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| Application images |  Immunofluorescence analysis of HeLa cells, using Histone H3 （Acetyl-Lys18） Antibody. The picture on the right is treated with the synthesized peptide.  Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue, using Histone H3 （Acetyl-Lys18） Antibody. The picture on the right is treated with the synthesized peptide.  Western blot analysis of extracts from HeLa cells, treated with TSA 400nM 24h, using Histone H3 （Acetyl-Lys18） Antibody. The lane on the right is treated with the synthesized peptide. |
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