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A0013 HSP90B (Phospho-Ser254) Antibody
点击次数：122发布时间：2012/9/12 0:35:56
更新日期：2024/10/16 16:00:07
所 在 地：美洲
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| Reactivity | Applications | MW. | Source |
| H, M, R | WB IHC IF ELISA | 83 kDa | Rabbit |
Note: The product is for research use only, not for use in diagnostic or therapeutic procedures.
| Product Name | HSP90B （Phospho-Ser254） Antibody |
| Synonyms | HS90B; HSP 84; HSP90-beta; HSP90AB1; HSPC2; HSPCB; Heat shock protein HSP 90-beta |
| Immunogen | The antiserum was produced against synthesized peptide derived from human HSP90B around the phosphorylation site of Ser254. |
| Immunogen Region | Internal |
| Clonality | Polyclonal |
| Modification sites | H:S254 M:S254 R:S254 |
| Application dilutions | WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:40000 |
| Specificity | HSP90B （Phospho-Ser254） Antibody detects endogenous levels of HSP90B only when phosphorylated at serine254. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation. |
| Physical Form | Rabbit IgG in phosphate buffered saline （without Mg2+ ａnd Ca2+）, pH 7.4, 150mM NaCl, 0.02% sodium azide ａnd 50% glycerol. |
| Concentration | 1 mg/ml |
| Storage/Stability | Store at -20℃/1 year |
| NCBI Gene Symbol | HSP90AB1 | ||||||||
| Database Links |
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| Application images |  Immunofluorescence analysis of HeLa cells treated with TNF-a 20nM 15＇, using HSP90B （Phospho-Ser254） Antibody. The picture on the right is treated with the synthesized peptide.  Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using HSP90B （Phospho-Ser254） Antibody. The picture on the right is treated with the synthesized peptide.  Western blot analysis of extracts from HeLa cells treated with TNF-a 10ng/ml 30＇, using HSP90B （Phospho-Ser254） Antibody. The lane on the right is treated with the synthesized peptide. |
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