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D0006 Histone H3 (Acetyl-Lys9) Antibody
点击次数:121发布时间:2012/9/12 14:36:55
更新日期:2024/10/16 16:00:19
所 在 地:美洲
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详细内容
Reactivity | Applications | MW. | Source |
H, M, R | WB IHC IF ELISA | 15 kDa | Rabbit |
Product Name | Histone H3 (Acetyl-Lys9) Antibody |
Synonyms | H3/a; H3/c; H3/d; H3/f; H3/h; H3/i; H3/j; H3/k; H3/l; H31; HIST1H3A; histone H3.1 |
Immunogen | The antiserum was produced against synthesized peptide derived from human Histone H3 around the acetylated site of Lys9. |
Immunogen Region | N-terminal |
Clonality | Polyclonal |
Modification sites | H:K9 M:K9 R:K9 |
Application dilutions | WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:1000 |
Specificity | Histone H3 (Acetyl-Lys9) Antibody detects endogenous levels of total Histone H3 protein only when acetylated at lysine9. |
Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific acetylated peptide. The antibody against non-acetylated peptide was removed by chromatography using non-acetylated peptide corresponding to the acetylation site. |
Physical Form | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Concentration | 1 mg/ml |
Storage/Stability | Store at -20℃/1 year |
NCBI Gene Symbol | HIST1H3A / HIST1H3B / HIST1H3C / HIST1H3D / HIST1H3E / HIST1H3F / HIST1H3G / HIST1H3H / HIST1H3I / HIST1H3J / HIST2H3A / HIST2H3C / HIST2H3D / H3F3A / H3F3B | ||||||||
Database Links |
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Application images | Immunofluorescence analysis of HeLa cells, using Histone H3 (Acetyl-Lys9) Antibody. The picture on the right is treated with the synthesized peptide. Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue, using Histone H3 (Acetyl-Lys9) Antibody. The picture on the right is treated with the synthesized peptide. Western blot analysis of extracts from Raw264.7 cells, treated with TSA 400nM 24h, using Histone H3 (Acetyl-Lys9) Antibody. The lane on the right is treated with the synthesized peptide. |
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