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D0006 Histone H3 (Acetyl-Lys9) Antibody

点击次数:121发布时间:2012/9/12 14:36:55

D0006  Histone H3 (Acetyl-Lys9) Antibody

更新日期:2024/10/16 16:00:19

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D0006  Histone H3 (Acetyl-Lys9) Antibody
Reactivity Applications MW. Source
H, M, R WB IHC IF ELISA 15 kDa Rabbit
Note: The product is for research use only, not for use in diagnostic or therapeutic procedures.
Product Name Histone H3 (Acetyl-Lys9) Antibody
Synonyms H3/a; H3/c; H3/d; H3/f; H3/h; H3/i; H3/j; H3/k; H3/l; H31; HIST1H3A; histone H3.1
Immunogen The antiserum was produced against synthesized peptide derived from human Histone H3 around the acetylated site of Lys9.
Immunogen Region N-terminal
Clonality Polyclonal
Modification sites H:K9 M:K9 R:K9
Application dilutions WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:1000
Specificity Histone H3 (Acetyl-Lys9) Antibody detects endogenous levels of total Histone H3 protein only when acetylated at lysine9.
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific acetylated peptide. The antibody against non-acetylated peptide was removed by chromatography using non-acetylated peptide corresponding to the acetylation site.
Physical Form Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration 1 mg/ml
Storage/Stability Store at -20℃/1 year
 
NCBI Gene Symbol HIST1H3A / HIST1H3B / HIST1H3C / HIST1H3D / HIST1H3E / HIST1H3F / HIST1H3G / HIST1H3H / HIST1H3I / HIST1H3J / HIST2H3A / HIST2H3C / HIST2H3D / H3F3A / H3F3B
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Application images
Histone H3 (Acetyl-Lys9) Antibody for Immunofluorescence

Immunofluorescence analysis of HeLa cells, using Histone H3 (Acetyl-Lys9) Antibody. The picture on the right is treated with the synthesized peptide.

Histone H3 (Acetyl-Lys9) Antibody for Immunohistochemistry

Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue, using Histone H3 (Acetyl-Lys9) Antibody. The picture on the right is treated with the synthesized peptide.

Histone H3 (Acetyl-Lys9) Antibody for Western Blot

Western blot analysis of extracts from Raw264.7 cells, treated with TSA 400nM 24h, using Histone H3 (Acetyl-Lys9) Antibody. The lane on the right is treated with the synthesized peptide.

 
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