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A7108 HNF4 alpha (Phospho-Ser313) Antibody
点击次数:176发布时间:2012/9/12 15:00:54
更新日期:2024/10/16 16:00:19
所 在 地:美洲
产品型号:
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详细内容
Reactivity | Applications | MW. | Source |
H, M, R | WB IHC IF ELISA | 52 kDa | Rabbit |
Product Name | HNF4 alpha (Phospho-Ser313) Antibody |
Synonyms | HNF-4; HNF-4-alpha; HNF4; HNF4A; Hepatocyte nuclear factor 4-alpha; NR2A1; TCF14; Transcription factor 14; Transcription factor HNF-4 |
Immunogen | The antiserum was produced against synthesized peptide derived from human HNF4 alpha around the phosphorylation site of Ser313. |
Immunogen Region | Internal |
Clonality | Polyclonal |
Modification sites | H:S313 M:S313 R:S313 |
Application dilutions | WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:20000 |
Specificity | HNF4 alpha (Phospho-Ser313) Antibody detects endogenous levels of HNF4 alpha only when phosphorylated at serine313. |
Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation. |
Physical Form | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Concentration | 1 mg/ml |
Storage/Stability | Store at -20℃/1 year |
NCBI Gene Symbol | HNF4A | ||||||||
Database Links |
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Application images | Immunofluorescence analysis of LOVO cells, using HNF4 alpha (Phospho-Ser313) Antibody. The picture on the right is treated with the synthesized peptide. Immunohistochemistry analysis of paraffin-embedded human liver carcinoma, using HNF4 alpha (Phospho-Ser313) Antibody. The picture on the right is treated with the synthesized peptide. Western blot analysis of extracts from HuvEc cells treated with EGF 200ng/ml 30', using HNF4 alpha (Phospho-Ser313) Antibody. The lane on the right is treated with the synthesized peptide. |
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