Product Name | HSP27 (Phospho-Ser78) Antibody |
Synonyms | 28 kDa heat shock protein; Estrogen-regulated 24 kDa protein; Growth-related 25 kDa protein; Heat shock 27 kDa protein; HSP 27; HSP25; HSPB1; P25; SRP27; Stress-responsive protein 27 |
Immunogen | The antiserum was produced against synthesized peptide derived from human HSP27 around the phosphorylation site of Ser78. |
Immunogen Region | Internal |
Clonality | Polyclonal |
Modification sites | H:S78 |
Application dilutions | WB: 1:500~1:1000 ELISA: 1:20000 |
Specificity | HSP27 (Phospho-Ser78) Antibody detects endogenous levels of HSP27 only when phosphorylated at serine78. |
Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation. |
Physical Form | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Concentration | 1 mg/ml |
Storage/Stability | Store at -20℃/1 year |