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A0013 HSP90B (Phospho-Ser254) Antibody
点击次数:134发布时间:2012/9/12 16:00:08
更新日期:2024/10/16 16:00:19
所 在 地:美洲
产品型号:
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详细内容
Reactivity | Applications | MW. | Source |
H, M, R | WB IHC IF ELISA | 83 kDa | Rabbit |
Product Name | HSP90B (Phospho-Ser254) Antibody |
Synonyms | HS90B; HSP 84; HSP90-beta; HSP90AB1; HSPC2; HSPCB; Heat shock protein HSP 90-beta |
Immunogen | The antiserum was produced against synthesized peptide derived from human HSP90B around the phosphorylation site of Ser254. |
Immunogen Region | Internal |
Clonality | Polyclonal |
Modification sites | H:S254 M:S254 R:S254 |
Application dilutions | WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:40000 |
Specificity | HSP90B (Phospho-Ser254) Antibody detects endogenous levels of HSP90B only when phosphorylated at serine254. |
Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation. |
Physical Form | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Concentration | 1 mg/ml |
Storage/Stability | Store at -20℃/1 year |
NCBI Gene Symbol | HSP90AB1 | ||||||||
Database Links |
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Application images | Immunofluorescence analysis of HeLa cells treated with TNF-a 20nM 15', using HSP90B (Phospho-Ser254) Antibody. The picture on the right is treated with the synthesized peptide. Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using HSP90B (Phospho-Ser254) Antibody. The picture on the right is treated with the synthesized peptide. Western blot analysis of extracts from HeLa cells treated with TNF-a 10ng/ml 30', using HSP90B (Phospho-Ser254) Antibody. The lane on the right is treated with the synthesized peptide. |
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