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A0013 HSP90B (Phospho-Ser254) Antibody

点击次数:134发布时间:2012/9/12 16:00:08

A0013  HSP90B (Phospho-Ser254) Antibody

更新日期:2024/10/16 16:00:19

所 在 地:美洲

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A0013  HSP90B (Phospho-Ser254) Antibody
Reactivity Applications MW. Source
H, M, R WB IHC IF ELISA 83 kDa Rabbit
Note: The product is for research use only, not for use in diagnostic or therapeutic procedures.
Product Name HSP90B (Phospho-Ser254) Antibody
Synonyms HS90B; HSP 84; HSP90-beta; HSP90AB1; HSPC2; HSPCB; Heat shock protein HSP 90-beta
Immunogen The antiserum was produced against synthesized peptide derived from human HSP90B around the phosphorylation site of Ser254.
Immunogen Region Internal
Clonality Polyclonal
Modification sites H:S254 M:S254 R:S254
Application dilutions WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:40000
Specificity HSP90B (Phospho-Ser254) Antibody detects endogenous levels of HSP90B only when phosphorylated at serine254.
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation.
Physical Form Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration 1 mg/ml
Storage/Stability Store at -20℃/1 year
 
NCBI Gene Symbol HSP90AB1
Database Links
Application images
HSP90B (Phospho-Ser254) Antibody for Immunofluorescence

Immunofluorescence analysis of HeLa cells treated with TNF-a 20nM 15', using HSP90B (Phospho-Ser254) Antibody. The picture on the right is treated with the synthesized peptide.

HSP90B (Phospho-Ser254) Antibody for Immunohistochemistry

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using HSP90B (Phospho-Ser254) Antibody. The picture on the right is treated with the synthesized peptide.

HSP90B (Phospho-Ser254) Antibody for Western Blot

Western blot analysis of extracts from HeLa cells treated with TNF-a 10ng/ml 30', using HSP90B (Phospho-Ser254) Antibody. The lane on the right is treated with the synthesized peptide.

 
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