产品展示
A1053 IkappaB-alpha (Phospho-Tyr305) Antibody
点击次数:113发布时间:2012/9/12 20:39:32
更新日期:2024/10/16 16:00:19
所 在 地:美洲
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详细内容
Reactivity | Applications | MW. | Source |
H, M, R | WB IHC ELISA | 35 kDa | Rabbit |
Product Name | IkappaB-alpha (Phospho-Tyr305) Antibody |
Synonyms | I-kappa-B-alpha; IkappaBalpha; IKBA; MAD3; Major histocompatibility complex enhancer-binding protein MAD3; NF-kappaB inhibitor alpha; NFKBI; NFKBIA; RL/IF-1 |
Immunogen | The antiserum was produced against synthesized peptide derived from human IkappaB-alpha around the phosphorylation site of Tyr305. |
Immunogen Region | C-terminal |
Clonality | Polyclonal |
Modification sites | H:Y305 M:Y302 R:Y302 |
Application dilutions | WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:1000 |
Specificity | IkappaB-alpha (Phospho-Tyr305) Antibody detects endogenous levels of IkappaB-alpha only when phosphorylated at tyrosine305. |
Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation. |
Physical Form | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Concentration | 1 mg/ml |
Storage/Stability | Store at -20℃/1 year |
NCBI Gene Symbol | NFKBIA | ||||||||
Database Links |
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Application images | Immunohistochemistry analysis of paraffin-embedded human lymph node, using IkappaB-alpha (Phospho-Tyr305) Antibody. The picture on the right is treated with the synthesized peptide. Western blot analysis of extracts from COS7 cells treated with nocodazole 1ug/ml 16h, using IkappaB-alpha (Phospho-Tyr305) Antibody. The lane on the right is treated with the synthesized peptide. |