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A0014 IkappaB-beta (Phospho-Ser23) Antibody
点击次数:202发布时间:2012/9/12 20:42:35
更新日期:2024/10/16 16:00:19
所 在 地:美洲
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详细内容
Reactivity | Applications | MW. | Source |
H, M, R | WB IHC IF ELISA | 37 kDa | Rabbit |
Product Name | IkappaB-beta (Phospho-Ser23) Antibody |
Synonyms | I-kappa-B-beta; IKB-B; IKBB; IkappaBbeta; NF-kappa-BIB; NF-kappaB inhibitor beta; NFKBIB, TR- interacting protein 9; TRIP9; Thyroid receptor interacting protein 9 |
Immunogen | The antiserum was produced against synthesized peptide derived from human IkappaB-beta around the phosphorylation site of Ser23. |
Immunogen Region | N-terminal |
Clonality | Polyclonal |
Modification sites | H:S23 M:S23 R:S23 |
Application dilutions | WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:40000 |
Specificity | IkappaB-beta (Phospho-Ser23) Antibody detects endogenous levels of IkappaB-beta only when phosphorylated at serine23. |
Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation. |
Physical Form | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Concentration | 1 mg/ml |
Storage/Stability | Store at -20℃/1 year |
NCBI Gene Symbol | NFKBIB | ||||||||
Database Links |
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Application images | Immunofluorescence analysis of HeLa cells treated with TNF-a 20nM 15', using IkappaB-beta (Phospho-Ser23) Antibody. The picture on the right is treated with the synthesized peptide. Immunohistochemistry analysis of paraffin-embedded human lung carcinoma, using IkappaB-beta (Phospho-Ser23) Antibody. The picture on the right is treated with the synthesized peptide. Western blot analysis of extracts from HeLa cells treated with TNF-a 20ng/ml 5', using IkappaB-beta (Phospho-Ser23) Antibody. The lane on the right is treated with the synthesized peptide. |
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