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A0441 IKK-alpha （Phospho-Ser176） /IKK-beta （Phospho-Ser177） Antibody
| Reactivity | Applications | MW. | Source |
| H, M, R | WB IHC ELISA | 84 kDa | Rabbit |
Note: The product is for research use only, not for use in diagnostic or therapeutic procedures.
| Product Name | IKK-alpha （Phospho-Ser176） /IKK-beta （Phospho-Ser177） Antibody |
| Synonyms | CHUK; Conserved helix-loop-helix ubiquitous kinase; I kappa-B kinase alpha; I-kappa-B kinase 1; IkappaB kinase; IkBKA; IKK-A; IKK-alpha; IKK1; IKKA; Inhibitor of nuclear factor kappa-B kinase alpha subunit; kinase IKK-alpha; NFKBIKA; Nuclear factor NFkappaB inhibitor kinase alpha; I-kappa-B kinase 2; I-kappa-B-kinase beta; IkBKB; IKK-B; IKK-beta; IKK2; IKKB; kinase IKK-beta; NFKBIKB; Nuclear factor NF-kappa-B inhibitor kinase beta |
| Immunogen | The antiserum was produced against synthesized peptide derived from human IKK-alpha around the phosphorylation site of Ser177. |
| Immunogen Region | Internal |
| Clonality | Polyclonal |
| Modification sites | H:S176/S177 M:S176/S177 R:S176/S177 |
| Application dilutions | WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:1000 |
| Specificity | IKK-alpha （Phospho-Ser176） /IKK-beta （Phospho-Ser177） Antibody detects endogenous levels of IKK-alpha only when phosphorylated at serine177. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation. |
| Physical Form | Rabbit IgG in phosphate buffered saline （without Mg2+ ａnd Ca2+）, pH 7.4, 150mM NaCl, 0.02% sodium azide ａnd 50% glycerol. |
| Concentration | 1 mg/ml |
| Storage/Stability | Store at -20℃/1 year |
| NCBI Gene Symbol | CHUK | ||||||||
| Database Links |
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| Application images |  Immunohistochemistry analysis of paraffin-embedded human heart, using IKK-alpha （Phospho-Ser176） /IKK-beta （Phospho-Ser177） Antibody. The picture on the right is treated with the synthesized peptide.  Western blot analysis of extracts from NIH-3T3 cells treated with TNF 20ng/ml 30＇, using IKK-alpha （Phospho-Ser176） /IKK-beta （Phospho-Ser177） Antibody. The lane on the right is treated with the synthesized peptide. |
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