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A7117 IKK-alpha (Phospho-Thr23) Antibody
点击次数:142发布时间:2012/9/12 20:55:05
更新日期:2024/10/16 16:00:19
所 在 地:美洲
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详细内容
Reactivity | Applications | MW. | Source |
H, M, R | WB IHC ELISA | 84 kDa | Rabbit |
Product Name | IKK-alpha (Phospho-Thr23) Antibody |
Synonyms | CHUK; Conserved helix-loop-helix ubiquitous kinase; I kappa-B kinase alpha; I-kappa-B kinase 1; IkappaB kinase; IkBKA; IKK-A; IKK-alpha; IKK1; IKKA; Inhibitor of nuclear factor kappa-B kinase alpha subunit; kinase IKK-alpha; NFKBIKA; Nuclear factor NFkappaB inhibitor kinase alpha |
Immunogen | The antiserum was produced against synthesized peptide derived from human IKK-alpha around the phosphorylation site of Thr23. |
Immunogen Region | N-terminal |
Clonality | Polyclonal |
Modification sites | H:T23 M:T23 R:T23 |
Application dilutions | WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:5000 |
Specificity | IKK-alpha (Phospho-Thr23) Antibody detects endogenous levels of IKK-alpha only when phosphorylated at threonine23. |
Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation. |
Physical Form | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Concentration | 1 mg/ml |
Storage/Stability | Store at -20℃/1 year |
NCBI Gene Symbol | CHUK | ||||||||
Database Links |
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Application images | Immunohistochemistry analysis of paraffin-embedded human colon carcinoma, using IKK-alpha (Phospho-Thr23) Antibody. The picture on the right is treated with the synthesized peptide. Western blot analysis of extracts from MDA-MB-435 cells treated with EGF, using IKK-alpha (Phospho-Thr23) Antibody. The lane on the left is treated with the synthesized peptide. |
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