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A0490 IKK-beta (Phospho-Tyr199) Antibody

点击次数:149发布时间:2012/9/12 21:04:17

A0490  IKK-beta (Phospho-Tyr199) Antibody

更新日期:2024/10/16 16:00:19

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A0490  IKK-beta (Phospho-Tyr199) Antibody
Reactivity Applications MW. Source
H, M, R WB IHC ELISA 86 kDa Rabbit
Note: The product is for research use only, not for use in diagnostic or therapeutic procedures.
Product Name IKK-beta (Phospho-Tyr199) Antibody
Synonyms I-kappa-B kinase 2; I-kappa-B-kinase beta; IKK-B; IKK-beta; IKK2; IKKB; IkBKB; NFKBIKB; Nuclear factor NF-kappa-B inhibitor kinase beta; kinase IKK-beta
Immunogen The antiserum was produced against synthesized peptide derived from human IKK-beta around the phosphorylation site of Tyr199.
Immunogen Region Internal
Clonality Polyclonal
Modification sites H:Y199 M:Y199 R:Y199
Application dilutions WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:5000
Specificity IKK-beta (Phospho-Tyr199) Antibody detects endogenous levels of IKK-beta only when phosphorylated at tyrosine199.
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation.
Physical Form Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration 1 mg/ml
Storage/Stability Store at -20℃/1 year
 
NCBI Gene Symbol IKBKB
Database Links
Application images
IKK-beta (Phospho-Tyr199) Antibody for Immunohistochemistry

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using IKK-beta (Phospho-Tyr199) Antibody. The picture on the right is treated with the synthesized peptide.

IKK-beta (Phospho-Tyr199) Antibody for Western Blot

Western blot analysis of extracts from HeLa cells treated with TNF-a 20ng/ml+Calyculin A 50nM 5', using IKK-beta (Phospho-Tyr199) Antibody. The lane on the right is treated with the synthesized peptide.

 
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