产品展示
A0490 IKK-beta (Phospho-Tyr199) Antibody
点击次数:149发布时间:2012/9/12 21:04:17
更新日期:2024/10/16 16:00:19
所 在 地:美洲
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详细内容
Reactivity | Applications | MW. | Source |
H, M, R | WB IHC ELISA | 86 kDa | Rabbit |
Product Name | IKK-beta (Phospho-Tyr199) Antibody |
Synonyms | I-kappa-B kinase 2; I-kappa-B-kinase beta; IKK-B; IKK-beta; IKK2; IKKB; IkBKB; NFKBIKB; Nuclear factor NF-kappa-B inhibitor kinase beta; kinase IKK-beta |
Immunogen | The antiserum was produced against synthesized peptide derived from human IKK-beta around the phosphorylation site of Tyr199. |
Immunogen Region | Internal |
Clonality | Polyclonal |
Modification sites | H:Y199 M:Y199 R:Y199 |
Application dilutions | WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:5000 |
Specificity | IKK-beta (Phospho-Tyr199) Antibody detects endogenous levels of IKK-beta only when phosphorylated at tyrosine199. |
Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation. |
Physical Form | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Concentration | 1 mg/ml |
Storage/Stability | Store at -20℃/1 year |
NCBI Gene Symbol | IKBKB | ||||||||
Database Links |
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Application images | Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using IKK-beta (Phospho-Tyr199) Antibody. The picture on the right is treated with the synthesized peptide. Western blot analysis of extracts from HeLa cells treated with TNF-a 20ng/ml+Calyculin A 50nM 5', using IKK-beta (Phospho-Tyr199) Antibody. The lane on the right is treated with the synthesized peptide. |
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