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重组大肠杆菌超氧化物歧化酶/SODA E.Coli
点击次数：158发布时间：2012/9/12 22:08:39
更新日期：2024/10/16 16:00:19
所 在 地：美洲
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重组大肠杆菌超氧化物歧化酶/SODA E.Coli
产品编号：PRO-208
产品规格：5ug/20ug/1mg
产品规格：5ug/20ug/1mg
| Catalogue Number | PRO-208 |
| Synonyms | Superoxide dismutase [Mn], MnSOD, soda, b3908, JW3879. |
| Introduction | Superoxide dismutase Mn （soda） belongs to the iron/manganese superoxide dismutase family. SodA destroys radicals that are typically produced within the cells ａnd which are toxic to biological systems. SodA works by catalyzing the dismutation of the superoxide radical O2- to O2 and H2O2, which are then metabolized to H2O ａnd O2 by catalase ａnd glutathione peroxidase. |
| Description | SODA produced in E.Coli is a single, non-glycosylated polypeptide chain containing 226 amino acids （1-206 a.a.） ａnd having a molecular mass of 25.2kDa. SODA is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques. |
| Source | Escherichia Coli. |
| Physical Appearance | Sterile filtered colorless solution. |
| Formulation | SODA E.coli solution （1mg/ml） containining 20mM Tris-HCl buffer （pH 8.0）, 1mM DTT, 10% glycerol ａnd 0.1M NaCl. |
| Stability | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein （0.1% HSA or BSA）. Avoid multiple freeze-thaw cycles. |
| Purity | Greater than 95% as determined by SDS-PAGE. |
| Amino acid sequence | MGSSHHHHHH SSGLVPRGSH MSYTLPSLPY AYDALEPHFD KQTMEIHHTK HHQTYVNNAN AALESLPEFA NLPVEELITK LDQLPADKKT VLRNNAGGHA NHSLFWKGLK KGTTLQGDLK AAIERDFGSV DNFKAEFEKA AASRFGSGWA WLVLKGDKLA VVSTANQDSP LMGEAISGAS GFPIMGLDVW EHAYYLKFQN RRPDYIKEFW NVVNWDEAAA RFAAKK. |
| Biological activity | Specific activity is > 350 units/mg, in which one unit will inhibit the rate of reduction of cytochrome c by 50% in a coupled system, using xanthine ａnd xanthine oxidase at pH 7.8 at 25C in a 1.5 ml reaction volume. |
| Usage | ProSpec＇s products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals. |
