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重组大鼠白细胞抑制因子/LIF Rat
点击次数：113发布时间：2012/9/13 14:05:47
更新日期：2024/10/16 16:00:19
所 在 地：美洲
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重组大鼠白细胞抑制因子/LIF Rat
产品编号：CYT-731
产品规格：2ug/10ug/1mg
产品规格：2ug/10ug/1mg
| Catalogue Number | CYT-731 |
| Synonyms | Leukemia inhibitory factor, Cholinergic neuronal differentiation factor, Lif. |
| Introduction | Leukemia Inhibitory Factor also called LIF is a lymphoid factor that promotes long-term maintenance of embryonic stem cells by suppressing spontaneous differentiation. Leukemia Inhibitory Factor has several functions such as cholinergic neuron differentiation, control of stem cell pluripotency, bone & fat metabolism, mitogenesis of factor dependent cell lines & promotion of megakaryocyte production in vivo. Human ａnd mouse LIF exhibit a 78% identity in its amino acid sequence. |
| Description | Leukemia Inhibitory Factor （LIF） Rat Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 180 amino acids ａnd having a molecular mass of 19.8 kDa. The Leukemia Inhibitory Factor （LIF） is purified by proprietary chromatographic techniques. |
| Source | Escherichia Coli. |
| Physical Appearance | Sterile Filtered White lyophilized （freeze-dried） powder. |
| Formulation | LIF Rat was lyophilized from 0.2μm filtered concentrated solution in 1xPBS, pH 7.4. |
| Solubility | It is recommended to reconstitute the lyophilized Leukemia Inhibitory Factor （LIF） in sterile water not less than 100μg/ml, which can then be further diluted to other aqueous solutions. |
| Stability | Lyophilized Leukemia Inhibitory Factor （LIF） although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Leukemia Inhibitory Factor （LIF） should be stored at 4°C between 2-7 days ａnd for future use below -18°C. For long term storage it is recommended to add a carrier protein （0.1% HSA or BSA）. Please prevent freeze-thaw cycles. |
| Purity | Greater than 96.0% as determined by: （a） Analysis by RP-HPLC. （b） Analysis by SDS-PAGE. |
| Amino acid sequence | SPLPITPVNA TCAIRHPCHG NLMNQIKSQL AQLNGSANAL FISYYTAQGE PFPNNVDKLC APNMTDFPPF HANGTEKTKL VELYRMVTYL GASLTNITWD QKNLNPTAVS LQIKLNATTD VMRGLLSSVL CRLCNKYHVG HVDVPCVPDN SSKEAFQRKK LGCQLLGTYK QVISVLAQAF . |
| Biological Activity | The activity of rat LIF is determined by the ability to induce differentiation of M1 myeloid leukemic cells. The minimum detectable concentration of rat LIF in this assay is 0.5ng/mL. |
| Usage | ProSpec＇s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals. |
