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重组人B细胞白血病/淋巴瘤2 （–BH3）/Bcl 2 Human （minus BH3 domain）
产品编号：PRO-633
产品规格：2μg/10μg/1mg
产品规格：2μg/10μg/1mg
| Catalogue Number | PRO-633 |
| Synonyms | Apoptosis regulator Bcl-2, BCL2, B-cell CLL/lymphoma 2, Bcl-2. |
| Introduction | BCL2 gene encodes an integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells such as lymphocytes. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma. Two transcript variants, produced by alternate splicing, differ in their C-terminal ends. |
| Description | Bcl-2 Des BH3 domain （93-107 residues） Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 203 amino acids 1-92 ａnd 108-218. The Bcl-2 is expressed as His-Tag fusion protein ａnd purified by proprietary chromatographic techniques. |
| Source | Escherichia Coli. |
| Physical Appearance | Sterile Filtered White lyophilized （freeze-dried） powder. |
| Formulation | The protein contains 10mM Tris-HCL pH-8, 1mM EDTA ａnd 250mM NaCl. |
| Solubility | Suspend Bcl-2 in 100?l of 0.5M Acetic acid, over night at 4°C. Dilute 10 fold into selected buffer system. BCL-2 has tendency to form intramolecular disulfide bond, 5mM DTT is recommended in assay buffer. When running SDS-PAGE gel, 10mM DTT is recommended. |
| Stability | Lyophilized Bcl-2 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Bcl-2 should be stored at 4°C between 2-7 days ａnd for future use below -18°C. For long term storage it is recommended to add a carrier protein （0.1% HSA or BSA）. Please prevent freeze-thaw cycles. |
| Purity | Greater than 95.0% as determined by（a） Analysis by RP-HPLC. （b） Analysis by SDS-PAGE. |
| Applications | Input marker or positive control （Western Blotting）. Function study. |
| Usage | ProSpec＇s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals. |
