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重组人胰蛋白酶/Trypsin Human
产品编号：PRO-770
产品规格：1mg/5mg/50mg
产品规格：1mg/5mg/50mg
| Catalogue Number | PRO-770 |
| Introduction | Trypsin （EC3.4.21.4） is part of the serine protease family. Trypsin cleaves lysine ａnd arginine at the C-terminal side of the peptide. The hydrolysis rate is slower if an acidic residue is on either sides of the cleavage site ａnd no cleavage occurs if a proline residue is on the carboxyl side of the cleavage site. Trypsin optimum pH is pH-7 to 9. Trypsin will also hydrolyze ester ａnd amide linkages of synthetic derivatives of amino acids such as: benzoyl L-arginine ethyl ester （BAEE）, p-toluenesulfonyl- L-arginine methyl ester （TAME）, tosyl-L-arginine methyl ester, N-α-benzoyl-L-arginine p-nitroanilide （BAPNA）, L-lysyl-p-nitroanilide, ａnd benzoyl-L-tyrosine ethyl ester （BTEE）. Serine protease inhibitors that inhibit recombinant trypsin include TLCK （N-p-tosyl-L-lysine chloromethyl ketone）, PMSF （phenylmethanesulfonyl fluoride）, benzamidine, soybean trypsin inhibitor, ａnd ovomucoid. |
| Description | Recombinant Human Trypsin-2 is free from any animal ａnd human sources. Recombinant Human Trypsin-2 expressed in E.Coli having a Mw of 24kDa is purified by standard chromatography techniques. Recombinant Human Trypsin-2 is free from foreign enzymes such as carboxypeptidase A & chymotrypsin. Recombinant Human Trypsin-2 is free from protease inhibitors such as PMSF ａnd EDTA. |
| Source | Escherichia Coli. |
| Physical Appearance | Sterile Filtered lyophilized powder. |
| Formulation | The protein was lyophilized with 10mM Sodium Acetate buffer ａnd 50mM NaCl. |
| Solubility | It is recommended to reconstitute the lyophilized Human Trypsin in sterile 18MΩ-cm H2O not less than 100μg/ml, which can then be further diluted to other aqueous solutions. |
| Stability | Recombinant Human Trypsin although stable at room temp for 1 week, should be stored desiccated below -18C. For long term storage it is recommended to add a carrier protein （0.1% HSA or BSA）. Please prevent freeze-thaw cycles. |
| Purity | Greater than 90% as determined by SDS-PAGE. |
| Unit Definition | One BAEE unit will produce a Delta A253 of 0.001 per minute in a reaction volume of 3.0ml at pH7.6 at 25C （1cm light path）. Trypsin digestion: suggested ratio （w:w） 1:20 to 1:1000 （Trypsin : Peptide）. |
| Biological Activity | 10,000 BAEE units/mg powder. |
| Usage | ProSpec＇s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals. |
