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重组猪胰岛素/Trypsin Porcine
产品编号：PRO-787
产品规格：1mg/2.5mg/50mg
产品规格：1mg/2.5mg/50mg
| Catalogue Number | PRO-787 |
| Introduction | Trypsin （EC3.4.21.4） is part of the serine protease family. Trypsin cleaves lysine ａnd arginine at the C-terminal side of the peptide. The hydrolysis rate is slower if an acidic residue is on either sides of the cleavage site ａnd no cleavage occurs if a proline residue is on the carboxyl side of the cleavage site. Trypsin optimum pH is pH-7 to 9. Trypsin will also hydrolyze ester ａnd amide linkages of synthetic derivatives of amino acids such as: benzoyl L-arginine ethyl ester （BAEE）, p-toluenesulfonyl- L-arginine methyl ester （TAME）, tosyl-L-arginine methyl ester, N-α-benzoyl-L-arginine p-nitroanilide （BAPNA）, L-lysyl-p-nitroanilide, ａnd benzoyl-L-tyrosine ethyl ester （BTEE）. Serine protease inhibitors that inhibit recombinant trypsin include TLCK （N-p-tosyl-L-lysine chloromethyl ketone）, PMSF （phenylmethanesulfonyl fluoride）, benzamidine, soybean trypsin inhibitor, ａnd ovomucoid. |
| Description | Recombinant Porcine Trypsin is free from any animal ａnd human sources. Recombinant Porcine Trypsin expressed in Yeast ａnd purified by standard chromatography techniques. Recombinant Porcine Trypsin is free from foreign enzymes such as carboxypeptidase A & chymotrypsin. Recombinant Human Trypsin is free from protease inhibitors such as PMSF ａnd EDTA. |
| Source | Pichia Pastoris. |
| Physical Appearance | Sterile Filtered clear liquid solution. |
| Formulation | The Porcine Trypsin （2.98mg/ml） is formulated with 1mM HCl ａnd 20mM CaCl2, pH-3. |
| Stability | Recombinant Porcine Trypsin should be stored at 2-8°C. For long term storage it is recommended to add a carrier protein （0.1% HSA or BSA）. |
| Biological Activity | 3,394 BAEE units/mg powder. |
| Amino Acid Sequence | VGGYTCAANSIPYQVSLNSGSHFCGGSLINSQWVVSAAHCYKSRIQVRLGEHNI DVLEGNEQFINAAKIITHPNFNGNTLDNDIMLIKLSSPATLNSRVATVSLPRSCA AAGTECLISGWGNTKSSGSSYPSLLQCLKAPVLSDSSCKSSYPGQITGNMICVGF LEGGKDSCQGDSGGPVVCNGQLQGIVSWGYGCAQKNKPGVYTKVCNYVNWI QQTIAAN. |
| Usage | ProSpec＇s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals. |
