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A0016 Keratin 18 (Phospho-Ser33) Antibody
点击次数:104发布时间:2012/9/13 15:53:08
更新日期:2024/10/16 16:00:19
所 在 地:美洲
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详细内容
Reactivity | Applications | MW. | Source |
H, M, R | WB IHC IF ELISA | 47 kDa | Rabbit |
Product Name | Keratin 18 (Phospho-Ser33) Antibody |
Synonyms | CK 18; CK18; CYK18; Cytokeratin endo B; K18; K1C18; KERD; KRT1-18; KRT18; Keratin; Keratin D; Keratin; type I cytoskeletal 18; cytokeratin 18; type I cytoskeletal 18 |
Immunogen | The antiserum was produced against synthesized peptide derived from human Keratin 18 around the phosphorylation site of Ser33. |
Immunogen Region | N-terminal |
Clonality | Polyclonal |
Modification sites | H:S34 M:S35 R:S35 |
Application dilutions | WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:5000 |
Specificity | Keratin 18 (Phospho-Ser33) Antibody detects endogenous levels of Keratin 18 only when phosphorylated at serine33. |
Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation. |
Physical Form | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Concentration | 1 mg/ml |
Storage/Stability | Store at -20℃/1 year |
NCBI Gene Symbol | KRT18 | ||||||||
Database Links |
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Application images | Immunofluorescence analysis of HeLa cells, using Keratin 18 (Phospho-Ser33) Antibody. The picture on the right is treated with the synthesized peptide. Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using Keratin 18 (Phospho-Ser33) Antibody. The picture on the right is treated with the synthesized peptide. Western blot analysis of extracts from HT29 cells, using Keratin 18 (Phospho-Ser33) Antibody. The lane on the right is treated with the synthesized peptide. |
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