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A0508 MDM2 (Phospho-Ser166) Antibody
点击次数：542发布时间：2012/9/14 9:46:01
更新日期：2024/10/16 16:00:19
所 在 地：美洲
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A0508 MDM2 （Phospho-Ser166） Antibody
| Reactivity | Applications | MW. | Source |
| H, M, | WB IHC IF ELISA | 55 kDa | Rabbit |
Note: The product is for research use only, not for use in diagnostic or therapeutic procedures.
| Product Name | MDM2 （Phospho-Ser166） Antibody |
| Synonyms | Double minute 2 protein; HDM2; Oncoprotein Mdm2; p53-binding protein Mdm2; Ubiquitin-protein ligase E3 Mdm2 |
| Immunogen | The antiserum was produced against synthesized peptide derived from human MDM2 around the phosphorylation site of Ser166. |
| Immunogen Region | Internal |
| Clonality | Polyclonal |
| Modification sites | H:S166 M:S163 |
| Application dilutions | WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:5000 |
| Specificity | MDM2 （Phospho-Ser166） Antibody detects endogenous levels of MDM2 only when phosphorylated at serine166. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation. |
| Physical Form | Rabbit IgG in phosphate buffered saline （without Mg2+ ａnd Ca2+）, pH 7.4, 150mM NaCl, 0.02% sodium azide ａnd 50% glycerol. |
| Concentration | 1 mg/ml |
| Storage/Stability | Store at -20℃/1 year |
| NCBI Gene Symbol | MDM2 | ||||||||
| Database Links |
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| Application images |  Immunofluorescence analysis of A549 cells, using MDM2 （Phospho-Ser166） Antibody. The picture on the right is treated with the synthesized peptide.  Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using MDM2 （Phospho-Ser166） Antibody. The picture on the right is treated with the synthesized peptide.  Western blot analysis of extracts from COS7 cells, using MDM2 （Phospho-Ser166） Antibody. The lane on the right is treated with the synthesized peptide. |
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