产品展示
A1154 MDM2 (Phospho-Ser186+Ser188) Antibody
点击次数:239发布时间:2012/9/14 9:54:14
更新日期:2024/10/16 16:00:19
所 在 地:美洲
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详细内容
Reactivity | Applications | MW. | Source |
H | IF ELISA | 55 kDa | Rabbit |
Product Name | MDM2 (Phospho-Ser186+Ser188) Antibody |
Synonyms | Double minute 2 protein; HDM2; Oncoprotein Mdm2; p53-binding protein Mdm2; Ubiquitin-protein ligase E3 Mdm2 |
Immunogen | The antiserum was produced against synthesized peptide derived from human MDM2 around the phosphorylation site of Ser186 and Ser188. |
Immunogen Region | Internal |
Clonality | Polyclonal |
Modification sites | H:S186+S188 |
Application dilutions | IF: 1:100~1:500 ELISA: 1:20000 |
Specificity | MDM2 (Phospho-Ser186+Ser188) Antibody detects endogenous levels of MDM2 only when phosphorylated at serine186 and serine188. |
Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation. |
Physical Form | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Concentration | 1 mg/ml |
Storage/Stability | Store at -20℃/1 year |
NCBI Gene Symbol | MDM2 | ||||||||
Database Links |
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Application images | Immunofluorescence analysis of HeLa cells, using MDM2 (Phospho-Ser186+Ser188) Antibody. The picture on the right is treated with the synthesized peptide. |