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A0681 MEK1 (Phospho-Thr286) Antibody
点击次数:137发布时间:2012/9/14 10:36:51
更新日期:2024/10/16 16:00:19
所 在 地:美洲
产品型号:
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详细内容
Reactivity | Applications | MW. | Source |
H, M, R | WB IHC ELISA | 43 kDa | Rabbit |
Product Name | MEK1 (Phospho-Thr286) Antibody |
Synonyms | Dual specificity mitogen-activated protein kinase kinase 1; ERK activator kinase 1; MAP kinase kinase 1; MAP2K1; MAPK/ERK kinase 1; MAPKK 1; MAPKK1; MEK1; MP2K1; PRKMK1; kinase MEK1 |
Immunogen | The antiserum was produced against synthesized peptide derived from human MEK1 around the phosphorylation site of Thr286. |
Immunogen Region | Internal |
Clonality | Polyclonal |
Modification sites | H:T286 M:T286 R:T286 |
Application dilutions | WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:40000 |
Specificity | MEK1 (Phospho-Thr286) Antibody detects endogenous levels of MEK1 only when phosphorylated at threonine286. |
Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation. |
Physical Form | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Concentration | 1 mg/ml |
Storage/Stability | Store at -20℃/1 year |
NCBI Gene Symbol | MAP2K1 | ||||||||
Database Links |
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Application images | Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using MEK1 (Phospho-Thr286) Antibody. The picture on the right is treated with the synthesized peptide. Western blot analysis of extracts from K562 cells, using MEK1 (Phospho-Thr286) Antibody. The lane on the right is treated with the synthesized peptide. |
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