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A7147 MEK1/2 (Phospho-Ser221) Antibody
点击次数：145发布时间：2012/9/14 10:49:51
更新日期：2024/10/16 16:00:19
所 在 地：美洲
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A7147 MEK1/2 （Phospho-Ser221） Antibody
| Reactivity | Applications | MW. | Source |
| H, M, R | WB IHC ELISA | 43 kDa | Rabbit |
Note: The product is for research use only, not for use in diagnostic or therapeutic procedures.
| Product Name | MEK1/2 （Phospho-Ser221） Antibody |
| Synonyms | Dual specificity mitogen-activated protein kinase kinase 1; ERK activator kinase 1; MAP kinase kinase 1; MAP2K1; MAPK/ERK kinase 1; MAPKK 1; MAPKK1; MEK1; MP2K1; PRKMK1; kinase MEK1 |
| Immunogen | The antiserum was produced against synthesized peptide derived from human MEK1/2 around the phosphorylation site of Ser221. |
| Immunogen Region | Internal |
| Clonality | Polyclonal |
| Modification sites | H:S222/226 M:S222/226 R:S222/226 |
| Application dilutions | WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:20000 |
| Specificity | MEK1/2 （Phospho-Ser221） Antibody detects endogenous levels of MEK1/2 only when phosphorylated at serine221. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation. |
| Physical Form | Rabbit IgG in phosphate buffered saline （without Mg2+ ａnd Ca2+）, pH 7.4, 150mM NaCl, 0.02% sodium azide ａnd 50% glycerol. |
| Concentration | 1 mg/ml |
| Storage/Stability | Store at -20℃/1 year |
| NCBI Gene Symbol | MAP2K2 | ||||||||
| Database Links |
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| Application images |  Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using MEK1/2 （Phospho-Ser221） Antibody. The picture on the right is treated with the synthesized peptide.  Western blot analysis of extracts from K562 cells treated with serum 20% 15＇ ａnd Jurkat cells treated with EGF, using MEK1/2 （Phospho-Ser221） Antibody. The lane on the right is treated with the synthesized peptide. |
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