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GMNN Human/重组人联会蛋白
产品编号：PRO-579
产品规格：5μg/20μg/1mg
Synonyms GMNN, Geminin, DNA Replication Inhibitor, Gem, RP3-369A17.3. Introduction Geminin is a 25 kDa nuclear protein, which inhibits DNA replication ａnd is degraded during the mitotic phase of the cell cycle. Geminin controls replication by binding to the licensing factor Cdt1, ａnd is involved in neural differentiation. In addition, Geminin directly interacts with Six3 ａnd Hox homeodomain proteins during embryogenesis ａnd inhibits their functions. Geminin can also promote DNA replication. Geminin has 2 roles in 2 different stages of the cell cycle: Geminin is a negative regulator of DNA replication during the "S phase" of the cell cycle. Inhibition of Geminin during the "S phase" （by RNAi） results in an additional round of replication of portions of the genome. During the "M phase" of the cell cycle （mitosis） Geminin stabilizes the replication factor Cdt1 promoting DNA replication during the next cell cycle. Moreover, inhibition of Geminin during mitosis （by RNAi） causes destabilization of Cdt1 protein ａnd impairment of DNA replication during the next cell cycle. Geminin thus guarantees that only one round of replication occurs during each cell cycle. It was discovered that Geminin is overexpressed in a number of malignancies ａnd cancer cell lines. This maintains the concept that Geminin has also a positive role in DNA replication ａnd cell cycle progression. Geminin accumulates through S, G2 ａnd M phases of the cell cycle but is absent during the G1 phase. During the metaphase/anaphase transition of mitosis Geminin levels decrease. Description Geminin Human Recombinant fused to N-terminal His-Tag produced in E.Coli is a single, non-glycosylated polypeptide chain containing 245 amino acids ａnd having a molecular mass of 27.7 kDa. Source Escherichia Coli. Physical Appearance Sterile Filtered colorless solution. Formulation The protein solution contains 20mM Tris pH 8, 100mM NaCl ａnd 10% glycerol. Stability Geminin although stable 4°C for 4 weeks, should be stored desiccated below -18°C.
For long term storage it is recommended to add a carrier protein （0.1% HSA or BSA）.
Please prevent freeze-thaw cycles. Amino Acid Sequence MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSMNPS MKQKQEEIKE NIKNSSVPRR TLKMIQPSAS GSLVGRENEL SAGLSKRKHR NDHLTSTTSS PGVIVPESSE NKNLGGVTQE SFDLMIKENP SSQYWKEVAE KRRKALYEAL KENEKLHKEI EQKDNEIARL KKENKELAEV AEHVQYMAEL IERLNGEPLD NFESLDNQEF DSEEETVEDS LVEDSEIGTC AEGTVSSSTD
AKPCI. Purity Greater than 95.0% as determined by（a） Analysis by RP-HPLC.
（b） Analysis by SDS-PAGE. Usage ProSpec＇s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
产品规格：5μg/20μg/1mg
| Synonyms | GMNN, Geminin, DNA Replication Inhibitor, Gem, RP3-369A17.3. |
| Introduction | Geminin is a 25 kDa nuclear protein, which inhibits DNA replication ａnd is degraded during the mitotic phase of the cell cycle. Geminin controls replication by binding to the licensing factor Cdt1, ａnd is involved in neural differentiation. In addition, Geminin directly interacts with Six3 ａnd Hox homeodomain proteins during embryogenesis ａnd inhibits their functions. Geminin can also promote DNA replication. Geminin has 2 roles in 2 different stages of the cell cycle: Geminin is a negative regulator of DNA replication during the "S phase" of the cell cycle. Inhibition of Geminin during the "S phase" （by RNAi） results in an additional round of replication of portions of the genome. During the "M phase" of the cell cycle （mitosis） Geminin stabilizes the replication factor Cdt1 promoting DNA replication during the next cell cycle. Moreover, inhibition of Geminin during mitosis （by RNAi） causes destabilization of Cdt1 protein ａnd impairment of DNA replication during the next cell cycle. Geminin thus guarantees that only one round of replication occurs during each cell cycle. It was discovered that Geminin is overexpressed in a number of malignancies ａnd cancer cell lines. This maintains the concept that Geminin has also a positive role in DNA replication ａnd cell cycle progression. Geminin accumulates through S, G2 ａnd M phases of the cell cycle but is absent during the G1 phase. During the metaphase/anaphase transition of mitosis Geminin levels decrease. |
| Description | Geminin Human Recombinant fused to N-terminal His-Tag produced in E.Coli is a single, non-glycosylated polypeptide chain containing 245 amino acids ａnd having a molecular mass of 27.7 kDa. |
| Source | Escherichia Coli. |
| Physical Appearance | Sterile Filtered colorless solution. |
| Formulation | The protein solution contains 20mM Tris pH 8, 100mM NaCl ａnd 10% glycerol. |
| Stability | Geminin although stable 4°C for 4 weeks, should be stored desiccated below -18°C. For long term storage it is recommended to add a carrier protein （0.1% HSA or BSA）. Please prevent freeze-thaw cycles. |
| Amino Acid Sequence | MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSMNPS MKQKQEEIKE NIKNSSVPRR TLKMIQPSAS GSLVGRENEL SAGLSKRKHR NDHLTSTTSS PGVIVPESSE NKNLGGVTQE SFDLMIKENP SSQYWKEVAE KRRKALYEAL KENEKLHKEI EQKDNEIARL KKENKELAEV AEHVQYMAEL IERLNGEPLD NFESLDNQEF DSEEETVEDS LVEDSEIGTC AEGTVSSSTD AKPCI. |
| Purity | Greater than 95.0% as determined by（a） Analysis by RP-HPLC. （b） Analysis by SDS-PAGE. |
| Usage | ProSpec＇s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals. |
