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DKK1 Human, HEK/重组人Dickkopf相关蛋白1, HEK
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DKK1 Human, HEK/重组人Dickkopf相关蛋白1, HEK
产品编号：PRO-815
产品规格：2μg/10μg/0.1mg
Synonyms DKK-1, DKK1, HDKK1, HDKK-1, Dickkopf-1, Dickkopf-related protein 1, SK. Introduction DKK1 is part of the dickkopf family. DKK1 is a secreted protein with 2 cysteine rich regions ａnd takes part in embryonic development through its inhibition of the WNT signaling pathway. High levels of DKK1 in bone marrow plasma ａnd peripheral blood is associated with the presence of osteolytic bone lesions in patients with multiple myeloma. Induction of DKK1 contributes to the pathological cascade triggered by beta-amyloid ａnd is significantly involved in the development of tau phosphorylation. DKK1 reduces the differentiation of osteoblastic precursor cells. DKK1 is an important member in multiple myeloma bone disease. DKK1 expression is reduced in human colon tumors, implying that DKK-1 plays a role as a tumor suppressor gene in this neoplasia; the Wnt/beta-catenin pathway is downregulated by the induction of DKK1 expression, a mechanism that is missing in colon cancer. Description DKK1 Human Recombinant produced in HEK-293 cells is a single, glycosylated, polypeptide chain containing 184 amino acids with a molecular weight of 20.7 kDa though on SDS-PAGE migrates at about 28kDa due to the glycosilation. The DKK1 is purified by proprietary chromatographic techniques. Source HEK293-F Cells. Physical Appearance Sterile Filtered White lyophilized （freeze-dried） powder. Formulation The DKK1 protein was lyophilized from a 0.2μm filtered solution containing no additives or preservatives. Solubility It is recommended to reconstitute the lyophilized DKK1 in sterile 18MΩ-cm H2O not less than 100μg/ml, which can then be further diluted to other aqueous solutions. Stability Lyophilized DKK1 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution DKK1 should be stored at 4°C between 2-7 days ａnd for future use below -18°C. For long term storage it is recommended to add a carrier protein （0.1% HSA or BSA）. Please prevent freeze-thaw cycles. Purity Greater than 95.0% as determined by SDS-PAGE. Amino acid sequence CTCVPPHPQT AFCNSDLVIR AKFVGTPEVN QTTLYQRYEI KMTKMYKGFQALGDAADIRF VYTPAMESVC GYFHRSHNRS EEFLIAGKLQ DGLLHITTCS FVAPWNSLSL AQRRGFTKTY TVGCEECTVF PCLSIPCKLQ SGTHCLWTDQ LLGSEKGFQ SRHLACLPRE PGLCTWQSLR SQIA. Biological Activity IC50 = 0.4 to 1.2 nM, determined by the concentration dependent inhibition of human MMP-2 hydrolysis of a peptide substrate （7-methoxycoumarin-4-yl）acetyl-Pro-Leu-Gly-Leu-（3-[2, 4-dinitrophenyl]-L-2, 3-diaminopropionyl）-Ala-Arg-NH2, under conditions in which recombinant pro-MMP-2 is activated, ａnd the peptide substrate concentration is 5μM. Usage ProSpec＇s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
DKK1 Human, HEK/重组人Dickkopf相关蛋白1, HEK
产品编号：PRO-815
产品规格：2μg/10μg/0.1mg
Synonyms DKK-1, DKK1, HDKK1, HDKK-1, Dickkopf-1, Dickkopf-related protein 1, SK. Introduction DKK1 is part of the dickkopf family. DKK1 is a secreted protein with 2 cysteine rich regions ａnd takes part in embryonic development through its inhibition of the WNT signaling pathway. High levels of DKK1 in bone marrow plasma ａnd peripheral blood is associated with the presence of osteolytic bone lesions in patients with multiple myeloma. Induction of DKK1 contributes to the pathological cascade triggered by beta-amyloid ａnd is significantly involved in the development of tau phosphorylation. DKK1 reduces the differentiation of osteoblastic precursor cells. DKK1 is an important member in multiple myeloma bone disease. DKK1 expression is reduced in human colon tumors, implying that DKK-1 plays a role as a tumor suppressor gene in this neoplasia; the Wnt/beta-catenin pathway is downregulated by the induction of DKK1 expression, a mechanism that is missing in colon cancer. Description DKK1 Human Recombinant produced in HEK-293 cells is a single, glycosylated, polypeptide chain containing 184 amino acids with a molecular weight of 20.7 kDa though on SDS-PAGE migrates at about 28kDa due to the glycosilation. The DKK1 is purified by proprietary chromatographic techniques. Source HEK293-F Cells. Physical Appearance Sterile Filtered White lyophilized （freeze-dried） powder. Formulation The DKK1 protein was lyophilized from a 0.2μm filtered solution containing no additives or preservatives. Solubility It is recommended to reconstitute the lyophilized DKK1 in sterile 18MΩ-cm H2O not less than 100μg/ml, which can then be further diluted to other aqueous solutions. Stability Lyophilized DKK1 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution DKK1 should be stored at 4°C between 2-7 days ａnd for future use below -18°C. For long term storage it is recommended to add a carrier protein （0.1% HSA or BSA）. Please prevent freeze-thaw cycles. Purity Greater than 95.0% as determined by SDS-PAGE. Amino acid sequence CTCVPPHPQT AFCNSDLVIR AKFVGTPEVN QTTLYQRYEI KMTKMYKGFQALGDAADIRF VYTPAMESVC GYFHRSHNRS EEFLIAGKLQ DGLLHITTCS FVAPWNSLSL AQRRGFTKTY TVGCEECTVF PCLSIPCKLQ SGTHCLWTDQ LLGSEKGFQ SRHLACLPRE PGLCTWQSLR SQIA. Biological Activity IC50 = 0.4 to 1.2 nM, determined by the concentration dependent inhibition of human MMP-2 hydrolysis of a peptide substrate （7-methoxycoumarin-4-yl）acetyl-Pro-Leu-Gly-Leu-（3-[2, 4-dinitrophenyl]-L-2, 3-diaminopropionyl）-Ala-Arg-NH2, under conditions in which recombinant pro-MMP-2 is activated, ａnd the peptide substrate concentration is 5μM. Usage ProSpec＇s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
DKK1 Human, HEK/重组人Dickkopf相关蛋白1, HEK
产品编号：PRO-815
产品规格：2μg/10μg/0.1mg
Synonyms DKK-1, DKK1, HDKK1, HDKK-1, Dickkopf-1, Dickkopf-related protein 1, SK. Introduction DKK1 is part of the dickkopf family. DKK1 is a secreted protein with 2 cysteine rich regions ａnd takes part in embryonic development through its inhibition of the WNT signaling pathway. High levels of DKK1 in bone marrow plasma ａnd peripheral blood is associated with the presence of osteolytic bone lesions in patients with multiple myeloma. Induction of DKK1 contributes to the pathological cascade triggered by beta-amyloid ａnd is significantly involved in the development of tau phosphorylation. DKK1 reduces the differentiation of osteoblastic precursor cells. DKK1 is an important member in multiple myeloma bone disease. DKK1 expression is reduced in human colon tumors, implying that DKK-1 plays a role as a tumor suppressor gene in this neoplasia; the Wnt/beta-catenin pathway is downregulated by the induction of DKK1 expression, a mechanism that is missing in colon cancer. Description DKK1 Human Recombinant produced in HEK-293 cells is a single, glycosylated, polypeptide chain containing 184 amino acids with a molecular weight of 20.7 kDa though on SDS-PAGE migrates at about 28kDa due to the glycosilation. The DKK1 is purified by proprietary chromatographic techniques. Source HEK293-F Cells. Physical Appearance Sterile Filtered White lyophilized （freeze-dried） powder. Formulation The DKK1 protein was lyophilized from a 0.2μm filtered solution containing no additives or preservatives. Solubility It is recommended to reconstitute the lyophilized DKK1 in sterile 18MΩ-cm H2O not less than 100μg/ml, which can then be further diluted to other aqueous solutions. Stability Lyophilized DKK1 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution DKK1 should be stored at 4°C between 2-7 days ａnd for future use below -18°C. For long term storage it is recommended to add a carrier protein （0.1% HSA or BSA）. Please prevent freeze-thaw cycles. Purity Greater than 95.0% as determined by SDS-PAGE. Amino acid sequence CTCVPPHPQT AFCNSDLVIR AKFVGTPEVN QTTLYQRYEI KMTKMYKGFQALGDAADIRF VYTPAMESVC GYFHRSHNRS EEFLIAGKLQ DGLLHITTCS FVAPWNSLSL AQRRGFTKTY TVGCEECTVF PCLSIPCKLQ SGTHCLWTDQ LLGSEKGFQ SRHLACLPRE PGLCTWQSLR SQIA. Biological Activity IC50 = 0.4 to 1.2 nM, determined by the concentration dependent inhibition of human MMP-2 hydrolysis of a peptide substrate （7-methoxycoumarin-4-yl）acetyl-Pro-Leu-Gly-Leu-（3-[2, 4-dinitrophenyl]-L-2, 3-diaminopropionyl）-Ala-Arg-NH2, under conditions in which recombinant pro-MMP-2 is activated, ａnd the peptide substrate concentration is 5μM. Usage ProSpec＇s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
DKK1 Human, HEK/重组人Dickkopf相关蛋白1, HEK
产品编号：PRO-815
产品规格：2μg/10μg/0.1mg
Synonyms DKK-1, DKK1, HDKK1, HDKK-1, Dickkopf-1, Dickkopf-related protein 1, SK. Introduction DKK1 is part of the dickkopf family. DKK1 is a secreted protein with 2 cysteine rich regions ａnd takes part in embryonic development through its inhibition of the WNT signaling pathway. High levels of DKK1 in bone marrow plasma ａnd peripheral blood is associated with the presence of osteolytic bone lesions in patients with multiple myeloma. Induction of DKK1 contributes to the pathological cascade triggered by beta-amyloid ａnd is significantly involved in the development of tau phosphorylation. DKK1 reduces the differentiation of osteoblastic precursor cells. DKK1 is an important member in multiple myeloma bone disease. DKK1 expression is reduced in human colon tumors, implying that DKK-1 plays a role as a tumor suppressor gene in this neoplasia; the Wnt/beta-catenin pathway is downregulated by the induction of DKK1 expression, a mechanism that is missing in colon cancer. Description DKK1 Human Recombinant produced in HEK-293 cells is a single, glycosylated, polypeptide chain containing 184 amino acids with a molecular weight of 20.7 kDa though on SDS-PAGE migrates at about 28kDa due to the glycosilation. The DKK1 is purified by proprietary chromatographic techniques. Source HEK293-F Cells. Physical Appearance Sterile Filtered White lyophilized （freeze-dried） powder. Formulation The DKK1 protein was lyophilized from a 0.2μm filtered solution containing no additives or preservatives. Solubility It is recommended to reconstitute the lyophilized DKK1 in sterile 18MΩ-cm H2O not less than 100μg/ml, which can then be further diluted to other aqueous solutions. Stability Lyophilized DKK1 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution DKK1 should be stored at 4°C between 2-7 days ａnd for future use below -18°C. For long term storage it is recommended to add a carrier protein （0.1% HSA or BSA）. Please prevent freeze-thaw cycles. Purity Greater than 95.0% as determined by SDS-PAGE. Amino acid sequence CTCVPPHPQT AFCNSDLVIR AKFVGTPEVN QTTLYQRYEI KMTKMYKGFQALGDAADIRF VYTPAMESVC GYFHRSHNRS EEFLIAGKLQ DGLLHITTCS FVAPWNSLSL AQRRGFTKTY TVGCEECTVF PCLSIPCKLQ SGTHCLWTDQ LLGSEKGFQ SRHLACLPRE PGLCTWQSLR SQIA. Biological Activity IC50 = 0.4 to 1.2 nM, determined by the concentration dependent inhibition of human MMP-2 hydrolysis of a peptide substrate （7-methoxycoumarin-4-yl）acetyl-Pro-Leu-Gly-Leu-（3-[2, 4-dinitrophenyl]-L-2, 3-diaminopropionyl）-Ala-Arg-NH2, under conditions in which recombinant pro-MMP-2 is activated, ａnd the peptide substrate concentration is 5μM. Usage ProSpec＇s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
DKK1 Human, HEK/重组人Dickkopf相关蛋白1, HEK
产品编号：PRO-815
产品规格：2μg/10μg/0.1mg
Synonyms DKK-1, DKK1, HDKK1, HDKK-1, Dickkopf-1, Dickkopf-related protein 1, SK. Introduction DKK1 is part of the dickkopf family. DKK1 is a secreted protein with 2 cysteine rich regions ａnd takes part in embryonic development through its inhibition of the WNT signaling pathway. High levels of DKK1 in bone marrow plasma ａnd peripheral blood is associated with the presence of osteolytic bone lesions in patients with multiple myeloma. Induction of DKK1 contributes to the pathological cascade triggered by beta-amyloid ａnd is significantly involved in the development of tau phosphorylation. DKK1 reduces the differentiation of osteoblastic precursor cells. DKK1 is an important member in multiple myeloma bone disease. DKK1 expression is reduced in human colon tumors, implying that DKK-1 plays a role as a tumor suppressor gene in this neoplasia; the Wnt/beta-catenin pathway is downregulated by the induction of DKK1 expression, a mechanism that is missing in colon cancer. Description DKK1 Human Recombinant produced in HEK-293 cells is a single, glycosylated, polypeptide chain containing 184 amino acids with a molecular weight of 20.7 kDa though on SDS-PAGE migrates at about 28kDa due to the glycosilation. The DKK1 is purified by proprietary chromatographic techniques. Source HEK293-F Cells. Physical Appearance Sterile Filtered White lyophilized （freeze-dried） powder. Formulation The DKK1 protein was lyophilized from a 0.2μm filtered solution containing no additives or preservatives. Solubility It is recommended to reconstitute the lyophilized DKK1 in sterile 18MΩ-cm H2O not less than 100μg/ml, which can then be further diluted to other aqueous solutions. Stability Lyophilized DKK1 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution DKK1 should be stored at 4°C between 2-7 days ａnd for future use below -18°C. For long term storage it is recommended to add a carrier protein （0.1% HSA or BSA）. Please prevent freeze-thaw cycles. Purity Greater than 95.0% as determined by SDS-PAGE. Amino acid sequence CTCVPPHPQT AFCNSDLVIR AKFVGTPEVN QTTLYQRYEI KMTKMYKGFQALGDAADIRF VYTPAMESVC GYFHRSHNRS EEFLIAGKLQ DGLLHITTCS FVAPWNSLSL AQRRGFTKTY TVGCEECTVF PCLSIPCKLQ SGTHCLWTDQ LLGSEKGFQ SRHLACLPRE PGLCTWQSLR SQIA. Biological Activity IC50 = 0.4 to 1.2 nM, determined by the concentration dependent inhibition of human MMP-2 hydrolysis of a peptide substrate （7-methoxycoumarin-4-yl）acetyl-Pro-Leu-Gly-Leu-（3-[2, 4-dinitrophenyl]-L-2, 3-diaminopropionyl）-Ala-Arg-NH2, under conditions in which recombinant pro-MMP-2 is activated, ａnd the peptide substrate concentration is 5μM. Usage ProSpec＇s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
DKK1 Human, HEK/重组人Dickkopf相关蛋白1, HEK
产品编号：PRO-815
产品规格：2μg/10μg/0.1mg
Synonyms DKK-1, DKK1, HDKK1, HDKK-1, Dickkopf-1, Dickkopf-related protein 1, SK. Introduction DKK1 is part of the dickkopf family. DKK1 is a secreted protein with 2 cysteine rich regions ａnd takes part in embryonic development through its inhibition of the WNT signaling pathway. High levels of DKK1 in bone marrow plasma ａnd peripheral blood is associated with the presence of osteolytic bone lesions in patients with multiple myeloma. Induction of DKK1 contributes to the pathological cascade triggered by beta-amyloid ａnd is significantly involved in the development of tau phosphorylation. DKK1 reduces the differentiation of osteoblastic precursor cells. DKK1 is an important member in multiple myeloma bone disease. DKK1 expression is reduced in human colon tumors, implying that DKK-1 plays a role as a tumor suppressor gene in this neoplasia; the Wnt/beta-catenin pathway is downregulated by the induction of DKK1 expression, a mechanism that is missing in colon cancer. Description DKK1 Human Recombinant produced in HEK-293 cells is a single, glycosylated, polypeptide chain containing 184 amino acids with a molecular weight of 20.7 kDa though on SDS-PAGE migrates at about 28kDa due to the glycosilation. The DKK1 is purified by proprietary chromatographic techniques. Source HEK293-F Cells. Physical Appearance Sterile Filtered White lyophilized （freeze-dried） powder. Formulation The DKK1 protein was lyophilized from a 0.2μm filtered solution containing no additives or preservatives. Solubility It is recommended to reconstitute the lyophilized DKK1 in sterile 18MΩ-cm H2O not less than 100μg/ml, which can then be further diluted to other aqueous solutions. Stability Lyophilized DKK1 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution DKK1 should be stored at 4°C between 2-7 days ａnd for future use below -18°C. For long term storage it is recommended to add a carrier protein （0.1% HSA or BSA）. Please prevent freeze-thaw cycles. Purity Greater than 95.0% as determined by SDS-PAGE. Amino acid sequence CTCVPPHPQT AFCNSDLVIR AKFVGTPEVN QTTLYQRYEI KMTKMYKGFQALGDAADIRF VYTPAMESVC GYFHRSHNRS EEFLIAGKLQ DGLLHITTCS FVAPWNSLSL AQRRGFTKTY TVGCEECTVF PCLSIPCKLQ SGTHCLWTDQ LLGSEKGFQ SRHLACLPRE PGLCTWQSLR SQIA. Biological Activity IC50 = 0.4 to 1.2 nM, determined by the concentration dependent inhibition of human MMP-2 hydrolysis of a peptide substrate （7-methoxycoumarin-4-yl）acetyl-Pro-Leu-Gly-Leu-（3-[2, 4-dinitrophenyl]-L-2, 3-diaminopropionyl）-Ala-Arg-NH2, under conditions in which recombinant pro-MMP-2 is activated, ａnd the peptide substrate concentration is 5μM. Usage ProSpec＇s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
DKK1 Human, HEK/重组人Dickkopf相关蛋白1, HEK
产品编号：PRO-815
产品规格：2μg/10μg/0.1mg
Synonyms DKK-1, DKK1, HDKK1, HDKK-1, Dickkopf-1, Dickkopf-related protein 1, SK. Introduction DKK1 is part of the dickkopf family. DKK1 is a secreted protein with 2 cysteine rich regions ａnd takes part in embryonic development through its inhibition of the WNT signaling pathway. High levels of DKK1 in bone marrow plasma ａnd peripheral blood is associated with the presence of osteolytic bone lesions in patients with multiple myeloma. Induction of DKK1 contributes to the pathological cascade triggered by beta-amyloid ａnd is significantly involved in the development of tau phosphorylation. DKK1 reduces the differentiation of osteoblastic precursor cells. DKK1 is an important member in multiple myeloma bone disease. DKK1 expression is reduced in human colon tumors, implying that DKK-1 plays a role as a tumor suppressor gene in this neoplasia; the Wnt/beta-catenin pathway is downregulated by the induction of DKK1 expression, a mechanism that is missing in colon cancer. Description DKK1 Human Recombinant produced in HEK-293 cells is a single, glycosylated, polypeptide chain containing 184 amino acids with a molecular weight of 20.7 kDa though on SDS-PAGE migrates at about 28kDa due to the glycosilation. The DKK1 is purified by proprietary chromatographic techniques. Source HEK293-F Cells. Physical Appearance Sterile Filtered White lyophilized （freeze-dried） powder. Formulation The DKK1 protein was lyophilized from a 0.2μm filtered solution containing no additives or preservatives. Solubility It is recommended to reconstitute the lyophilized DKK1 in sterile 18MΩ-cm H2O not less than 100μg/ml, which can then be further diluted to other aqueous solutions. Stability Lyophilized DKK1 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution DKK1 should be stored at 4°C between 2-7 days ａnd for future use below -18°C. For long term storage it is recommended to add a carrier protein （0.1% HSA or BSA）. Please prevent freeze-thaw cycles. Purity Greater than 95.0% as determined by SDS-PAGE. Amino acid sequence CTCVPPHPQT AFCNSDLVIR AKFVGTPEVN QTTLYQRYEI KMTKMYKGFQALGDAADIRF VYTPAMESVC GYFHRSHNRS EEFLIAGKLQ DGLLHITTCS FVAPWNSLSL AQRRGFTKTY TVGCEECTVF PCLSIPCKLQ SGTHCLWTDQ LLGSEKGFQ SRHLACLPRE PGLCTWQSLR SQIA. Biological Activity IC50 = 0.4 to 1.2 nM, determined by the concentration dependent inhibition of human MMP-2 hydrolysis of a peptide substrate （7-methoxycoumarin-4-yl）acetyl-Pro-Leu-Gly-Leu-（3-[2, 4-dinitrophenyl]-L-2, 3-diaminopropionyl）-Ala-Arg-NH2, under conditions in which recombinant pro-MMP-2 is activated, ａnd the peptide substrate concentration is 5μM. Usage ProSpec＇s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
DKK1 Human, HEK/重组人Dickkopf相关蛋白1, HEK
产品编号：PRO-815
产品规格：2μg/10μg/0.1mg
Synonyms DKK-1, DKK1, HDKK1, HDKK-1, Dickkopf-1, Dickkopf-related protein 1, SK. Introduction DKK1 is part of the dickkopf family. DKK1 is a secreted protein with 2 cysteine rich regions ａnd takes part in embryonic development through its inhibition of the WNT signaling pathway. High levels of DKK1 in bone marrow plasma ａnd peripheral blood is associated with the presence of osteolytic bone lesions in patients with multiple myeloma. Induction of DKK1 contributes to the pathological cascade triggered by beta-amyloid ａnd is significantly involved in the development of tau phosphorylation. DKK1 reduces the differentiation of osteoblastic precursor cells. DKK1 is an important member in multiple myeloma bone disease. DKK1 expression is reduced in human colon tumors, implying that DKK-1 plays a role as a tumor suppressor gene in this neoplasia; the Wnt/beta-catenin pathway is downregulated by the induction of DKK1 expression, a mechanism that is missing in colon cancer. Description DKK1 Human Recombinant produced in HEK-293 cells is a single, glycosylated, polypeptide chain containing 184 amino acids with a molecular weight of 20.7 kDa though on SDS-PAGE migrates at about 28kDa due to the glycosilation. The DKK1 is purified by proprietary chromatographic techniques. Source HEK293-F Cells. Physical Appearance Sterile Filtered White lyophilized （freeze-dried） powder. Formulation The DKK1 protein was lyophilized from a 0.2μm filtered solution containing no additives or preservatives. Solubility It is recommended to reconstitute the lyophilized DKK1 in sterile 18MΩ-cm H2O not less than 100μg/ml, which can then be further diluted to other aqueous solutions. Stability Lyophilized DKK1 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution DKK1 should be stored at 4°C between 2-7 days ａnd for future use below -18°C. For long term storage it is recommended to add a carrier protein （0.1% HSA or BSA）. Please prevent freeze-thaw cycles. Purity Greater than 95.0% as determined by SDS-PAGE. Amino acid sequence CTCVPPHPQT AFCNSDLVIR AKFVGTPEVN QTTLYQRYEI KMTKMYKGFQALGDAADIRF VYTPAMESVC GYFHRSHNRS EEFLIAGKLQ DGLLHITTCS FVAPWNSLSL AQRRGFTKTY TVGCEECTVF PCLSIPCKLQ SGTHCLWTDQ LLGSEKGFQ SRHLACLPRE PGLCTWQSLR SQIA. Biological Activity IC50 = 0.4 to 1.2 nM, determined by the concentration dependent inhibition of human MMP-2 hydrolysis of a peptide substrate （7-methoxycoumarin-4-yl）acetyl-Pro-Leu-Gly-Leu-（3-[2, 4-dinitrophenyl]-L-2, 3-diaminopropionyl）-Ala-Arg-NH2, under conditions in which recombinant pro-MMP-2 is activated, ａnd the peptide substrate concentration is 5μM. Usage ProSpec＇s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
DKK1 Human, HEK/重组人Dickkopf相关蛋白1, HEK
产品编号：PRO-815
产品规格：2μg/10μg/0.1mg
Synonyms DKK-1, DKK1, HDKK1, HDKK-1, Dickkopf-1, Dickkopf-related protein 1, SK. Introduction DKK1 is part of the dickkopf family. DKK1 is a secreted protein with 2 cysteine rich regions ａnd takes part in embryonic development through its inhibition of the WNT signaling pathway. High levels of DKK1 in bone marrow plasma ａnd peripheral blood is associated with the presence of osteolytic bone lesions in patients with multiple myeloma. Induction of DKK1 contributes to the pathological cascade triggered by beta-amyloid ａnd is significantly involved in the development of tau phosphorylation. DKK1 reduces the differentiation of osteoblastic precursor cells. DKK1 is an important member in multiple myeloma bone disease. DKK1 expression is reduced in human colon tumors, implying that DKK-1 plays a role as a tumor suppressor gene in this neoplasia; the Wnt/beta-catenin pathway is downregulated by the induction of DKK1 expression, a mechanism that is missing in colon cancer. Description DKK1 Human Recombinant produced in HEK-293 cells is a single, glycosylated, polypeptide chain containing 184 amino acids with a molecular weight of 20.7 kDa though on SDS-PAGE migrates at about 28kDa due to the glycosilation. The DKK1 is purified by proprietary chromatographic techniques. Source HEK293-F Cells. Physical Appearance Sterile Filtered White lyophilized （freeze-dried） powder. Formulation The DKK1 protein was lyophilized from a 0.2μm filtered solution containing no additives or preservatives. Solubility It is recommended to reconstitute the lyophilized DKK1 in sterile 18MΩ-cm H2O not less than 100μg/ml, which can then be further diluted to other aqueous solutions. Stability Lyophilized DKK1 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution DKK1 should be stored at 4°C between 2-7 days ａnd for future use below -18°C. For long term storage it is recommended to add a carrier protein （0.1% HSA or BSA）. Please prevent freeze-thaw cycles. Purity Greater than 95.0% as determined by SDS-PAGE. Amino acid sequence CTCVPPHPQT AFCNSDLVIR AKFVGTPEVN QTTLYQRYEI KMTKMYKGFQALGDAADIRF VYTPAMESVC GYFHRSHNRS EEFLIAGKLQ DGLLHITTCS FVAPWNSLSL AQRRGFTKTY TVGCEECTVF PCLSIPCKLQ SGTHCLWTDQ LLGSEKGFQ SRHLACLPRE PGLCTWQSLR SQIA. Biological Activity IC50 = 0.4 to 1.2 nM, determined by the concentration dependent inhibition of human MMP-2 hydrolysis of a peptide substrate （7-methoxycoumarin-4-yl）acetyl-Pro-Leu-Gly-Leu-（3-[2, 4-dinitrophenyl]-L-2, 3-diaminopropionyl）-Ala-Arg-NH2, under conditions in which recombinant pro-MMP-2 is activated, ａnd the peptide substrate concentration is 5μM. Usage ProSpec＇s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
DKK1 Human, HEK/重组人Dickkopf相关蛋白1, HEK
产品编号：PRO-815
产品规格：2μg/10μg/0.1mg
Synonyms DKK-1, DKK1, HDKK1, HDKK-1, Dickkopf-1, Dickkopf-related protein 1, SK. Introduction DKK1 is part of the dickkopf family. DKK1 is a secreted protein with 2 cysteine rich regions ａnd takes part in embryonic development through its inhibition of the WNT signaling pathway. High levels of DKK1 in bone marrow plasma ａnd peripheral blood is associated with the presence of osteolytic bone lesions in patients with multiple myeloma. Induction of DKK1 contributes to the pathological cascade triggered by beta-amyloid ａnd is significantly involved in the development of tau phosphorylation. DKK1 reduces the differentiation of osteoblastic precursor cells. DKK1 is an important member in multiple myeloma bone disease. DKK1 expression is reduced in human colon tumors, implying that DKK-1 plays a role as a tumor suppressor gene in this neoplasia; the Wnt/beta-catenin pathway is downregulated by the induction of DKK1 expression, a mechanism that is missing in colon cancer. Description DKK1 Human Recombinant produced in HEK-293 cells is a single, glycosylated, polypeptide chain containing 184 amino acids with a molecular weight of 20.7 kDa though on SDS-PAGE migrates at about 28kDa due to the glycosilation. The DKK1 is purified by proprietary chromatographic techniques. Source HEK293-F Cells. Physical Appearance Sterile Filtered White lyophilized （freeze-dried） powder. Formulation The DKK1 protein was lyophilized from a 0.2μm filtered solution containing no additives or preservatives. Solubility It is recommended to reconstitute the lyophilized DKK1 in sterile 18MΩ-cm H2O not less than 100μg/ml, which can then be further diluted to other aqueous solutions. Stability Lyophilized DKK1 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution DKK1 should be stored at 4°C between 2-7 days ａnd for future use below -18°C. For long term storage it is recommended to add a carrier protein （0.1% HSA or BSA）. Please prevent freeze-thaw cycles. Purity Greater than 95.0% as determined by SDS-PAGE. Amino acid sequence CTCVPPHPQT AFCNSDLVIR AKFVGTPEVN QTTLYQRYEI KMTKMYKGFQALGDAADIRF VYTPAMESVC GYFHRSHNRS EEFLIAGKLQ DGLLHITTCS FVAPWNSLSL AQRRGFTKTY TVGCEECTVF PCLSIPCKLQ SGTHCLWTDQ LLGSEKGFQ SRHLACLPRE PGLCTWQSLR SQIA. Biological Activity IC50 = 0.4 to 1.2 nM, determined by the concentration dependent inhibition of human MMP-2 hydrolysis of a peptide substrate （7-methoxycoumarin-4-yl）acetyl-Pro-Leu-Gly-Leu-（3-[2, 4-dinitrophenyl]-L-2, 3-diaminopropionyl）-Ala-Arg-NH2, under conditions in which recombinant pro-MMP-2 is activated, ａnd the peptide substrate concentration is 5μM. Usage ProSpec＇s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
DKK1 Human, HEK/重组人Dickkopf相关蛋白1, HEK
产品编号：PRO-815
产品规格：2μg/10μg/0.1mg
Synonyms DKK-1, DKK1, HDKK1, HDKK-1, Dickkopf-1, Dickkopf-related protein 1, SK. Introduction DKK1 is part of the dickkopf family. DKK1 is a secreted protein with 2 cysteine rich regions ａnd takes part in embryonic development through its inhibition of the WNT signaling pathway. High levels of DKK1 in bone marrow plasma ａnd peripheral blood is associated with the presence of osteolytic bone lesions in patients with multiple myeloma. Induction of DKK1 contributes to the pathological cascade triggered by beta-amyloid ａnd is significantly involved in the development of tau phosphorylation. DKK1 reduces the differentiation of osteoblastic precursor cells. DKK1 is an important member in multiple myeloma bone disease. DKK1 expression is reduced in human colon tumors, implying that DKK-1 plays a role as a tumor suppressor gene in this neoplasia; the Wnt/beta-catenin pathway is downregulated by the induction of DKK1 expression, a mechanism that is missing in colon cancer. Description DKK1 Human Recombinant produced in HEK-293 cells is a single, glycosylated, polypeptide chain containing 184 amino acids with a molecular weight of 20.7 kDa though on SDS-PAGE migrates at about 28kDa due to the glycosilation. The DKK1 is purified by proprietary chromatographic techniques. Source HEK293-F Cells. Physical Appearance Sterile Filtered White lyophilized （freeze-dried） powder. Formulation The DKK1 protein was lyophilized from a 0.2μm filtered solution containing no additives or preservatives. Solubility It is recommended to reconstitute the lyophilized DKK1 in sterile 18MΩ-cm H2O not less than 100μg/ml, which can then be further diluted to other aqueous solutions. Stability Lyophilized DKK1 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution DKK1 should be stored at 4°C between 2-7 days ａnd for future use below -18°C. For long term storage it is recommended to add a carrier protein （0.1% HSA or BSA）. Please prevent freeze-thaw cycles. Purity Greater than 95.0% as determined by SDS-PAGE. Amino acid sequence CTCVPPHPQT AFCNSDLVIR AKFVGTPEVN QTTLYQRYEI KMTKMYKGFQALGDAADIRF VYTPAMESVC GYFHRSHNRS EEFLIAGKLQ DGLLHITTCS FVAPWNSLSL AQRRGFTKTY TVGCEECTVF PCLSIPCKLQ SGTHCLWTDQ LLGSEKGFQ SRHLACLPRE PGLCTWQSLR SQIA. Biological Activity IC50 = 0.4 to 1.2 nM, determined by the concentration dependent inhibition of human MMP-2 hydrolysis of a peptide substrate （7-methoxycoumarin-4-yl）acetyl-Pro-Leu-Gly-Leu-（3-[2, 4-dinitrophenyl]-L-2, 3-diaminopropionyl）-Ala-Arg-NH2, under conditions in which recombinant pro-MMP-2 is activated, ａnd the peptide substrate concentration is 5μM. Usage ProSpec＇s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
DKK1 Human, HEK/重组人Dickkopf相关蛋白1, HEK
产品编号：PRO-815
产品规格：2μg/10μg/0.1mg
Synonyms DKK-1, DKK1, HDKK1, HDKK-1, Dickkopf-1, Dickkopf-related protein 1, SK. Introduction DKK1 is part of the dickkopf family. DKK1 is a secreted protein with 2 cysteine rich regions ａnd takes part in embryonic development through its inhibition of the WNT signaling pathway. High levels of DKK1 in bone marrow plasma ａnd peripheral blood is associated with the presence of osteolytic bone lesions in patients with multiple myeloma. Induction of DKK1 contributes to the pathological cascade triggered by beta-amyloid ａnd is significantly involved in the development of tau phosphorylation. DKK1 reduces the differentiation of osteoblastic precursor cells. DKK1 is an important member in multiple myeloma bone disease. DKK1 expression is reduced in human colon tumors, implying that DKK-1 plays a role as a tumor suppressor gene in this neoplasia; the Wnt/beta-catenin pathway is downregulated by the induction of DKK1 expression, a mechanism that is missing in colon cancer. Description DKK1 Human Recombinant produced in HEK-293 cells is a single, glycosylated, polypeptide chain containing 184 amino acids with a molecular weight of 20.7 kDa though on SDS-PAGE migrates at about 28kDa due to the glycosilation. The DKK1 is purified by proprietary chromatographic techniques. Source HEK293-F Cells. Physical Appearance Sterile Filtered White lyophilized （freeze-dried） powder. Formulation The DKK1 protein was lyophilized from a 0.2μm filtered solution containing no additives or preservatives. Solubility It is recommended to reconstitute the lyophilized DKK1 in sterile 18MΩ-cm H2O not less than 100μg/ml, which can then be further diluted to other aqueous solutions. Stability Lyophilized DKK1 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution DKK1 should be stored at 4°C between 2-7 days ａnd for future use below -18°C. For long term storage it is recommended to add a carrier protein （0.1% HSA or BSA）. Please prevent freeze-thaw cycles. Purity Greater than 95.0% as determined by SDS-PAGE. Amino acid sequence CTCVPPHPQT AFCNSDLVIR AKFVGTPEVN QTTLYQRYEI KMTKMYKGFQALGDAADIRF VYTPAMESVC GYFHRSHNRS EEFLIAGKLQ DGLLHITTCS FVAPWNSLSL AQRRGFTKTY TVGCEECTVF PCLSIPCKLQ SGTHCLWTDQ LLGSEKGFQ SRHLACLPRE PGLCTWQSLR SQIA. Biological Activity IC50 = 0.4 to 1.2 nM, determined by the concentration dependent inhibition of human MMP-2 hydrolysis of a peptide substrate （7-methoxycoumarin-4-yl）acetyl-Pro-Leu-Gly-Leu-（3-[2, 4-dinitrophenyl]-L-2, 3-diaminopropionyl）-Ala-Arg-NH2, under conditions in which recombinant pro-MMP-2 is activated, ａnd the peptide substrate concentration is 5μM. Usage ProSpec＇s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
DKK1 Human, HEK/重组人Dickkopf相关蛋白1, HEK
产品编号：PRO-815
产品规格：2μg/10μg/0.1mg
Synonyms DKK-1, DKK1, HDKK1, HDKK-1, Dickkopf-1, Dickkopf-related protein 1, SK. Introduction DKK1 is part of the dickkopf family. DKK1 is a secreted protein with 2 cysteine rich regions ａnd takes part in embryonic development through its inhibition of the WNT signaling pathway. High levels of DKK1 in bone marrow plasma ａnd peripheral blood is associated with the presence of osteolytic bone lesions in patients with multiple myeloma. Induction of DKK1 contributes to the pathological cascade triggered by beta-amyloid ａnd is significantly involved in the development of tau phosphorylation. DKK1 reduces the differentiation of osteoblastic precursor cells. DKK1 is an important member in multiple myeloma bone disease. DKK1 expression is reduced in human colon tumors, implying that DKK-1 plays a role as a tumor suppressor gene in this neoplasia; the Wnt/beta-catenin pathway is downregulated by the induction of DKK1 expression, a mechanism that is missing in colon cancer. Description DKK1 Human Recombinant produced in HEK-293 cells is a single, glycosylated, polypeptide chain containing 184 amino acids with a molecular weight of 20.7 kDa though on SDS-PAGE migrates at about 28kDa due to the glycosilation. The DKK1 is purified by proprietary chromatographic techniques. Source HEK293-F Cells. Physical Appearance Sterile Filtered White lyophilized （freeze-dried） powder. Formulation The DKK1 protein was lyophilized from a 0.2μm filtered solution containing no additives or preservatives. Solubility It is recommended to reconstitute the lyophilized DKK1 in sterile 18MΩ-cm H2O not less than 100μg/ml, which can then be further diluted to other aqueous solutions. Stability Lyophilized DKK1 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution DKK1 should be stored at 4°C between 2-7 days ａnd for future use below -18°C. For long term storage it is recommended to add a carrier protein （0.1% HSA or BSA）. Please prevent freeze-thaw cycles. Purity Greater than 95.0% as determined by SDS-PAGE. Amino acid sequence CTCVPPHPQT AFCNSDLVIR AKFVGTPEVN QTTLYQRYEI KMTKMYKGFQALGDAADIRF VYTPAMESVC GYFHRSHNRS EEFLIAGKLQ DGLLHITTCS FVAPWNSLSL AQRRGFTKTY TVGCEECTVF PCLSIPCKLQ SGTHCLWTDQ LLGSEKGFQ SRHLACLPRE PGLCTWQSLR SQIA. Biological Activity IC50 = 0.4 to 1.2 nM, determined by the concentration dependent inhibition of human MMP-2 hydrolysis of a peptide substrate （7-methoxycoumarin-4-yl）acetyl-Pro-Leu-Gly-Leu-（3-[2, 4-dinitrophenyl]-L-2, 3-diaminopropionyl）-Ala-Arg-NH2, under conditions in which recombinant pro-MMP-2 is activated, ａnd the peptide substrate concentration is 5μM. Usage ProSpec＇s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
DKK1 Human, HEK/重组人Dickkopf相关蛋白1, HEK
产品编号：PRO-815
产品规格：2μg/10μg/0.1mg
Synonyms DKK-1, DKK1, HDKK1, HDKK-1, Dickkopf-1, Dickkopf-related protein 1, SK. Introduction DKK1 is part of the dickkopf family. DKK1 is a secreted protein with 2 cysteine rich regions ａnd takes part in embryonic development through its inhibition of the WNT signaling pathway. High levels of DKK1 in bone marrow plasma ａnd peripheral blood is associated with the presence of osteolytic bone lesions in patients with multiple myeloma. Induction of DKK1 contributes to the pathological cascade triggered by beta-amyloid ａnd is significantly involved in the development of tau phosphorylation. DKK1 reduces the differentiation of osteoblastic precursor cells. DKK1 is an important member in multiple myeloma bone disease. DKK1 expression is reduced in human colon tumors, implying that DKK-1 plays a role as a tumor suppressor gene in this neoplasia; the Wnt/beta-catenin pathway is downregulated by the induction of DKK1 expression, a mechanism that is missing in colon cancer. Description DKK1 Human Recombinant produced in HEK-293 cells is a single, glycosylated, polypeptide chain containing 184 amino acids with a molecular weight of 20.7 kDa though on SDS-PAGE migrates at about 28kDa due to the glycosilation. The DKK1 is purified by proprietary chromatographic techniques. Source HEK293-F Cells. Physical Appearance Sterile Filtered White lyophilized （freeze-dried） powder. Formulation The DKK1 protein was lyophilized from a 0.2μm filtered solution containing no additives or preservatives. Solubility It is recommended to reconstitute the lyophilized DKK1 in sterile 18MΩ-cm H2O not less than 100μg/ml, which can then be further diluted to other aqueous solutions. Stability Lyophilized DKK1 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution DKK1 should be stored at 4°C between 2-7 days ａnd for future use below -18°C. For long term storage it is recommended to add a carrier protein （0.1% HSA or BSA）. Please prevent freeze-thaw cycles. Purity Greater than 95.0% as determined by SDS-PAGE. Amino acid sequence CTCVPPHPQT AFCNSDLVIR AKFVGTPEVN QTTLYQRYEI KMTKMYKGFQALGDAADIRF VYTPAMESVC GYFHRSHNRS EEFLIAGKLQ DGLLHITTCS FVAPWNSLSL AQRRGFTKTY TVGCEECTVF PCLSIPCKLQ SGTHCLWTDQ LLGSEKGFQ SRHLACLPRE PGLCTWQSLR SQIA. Biological Activity IC50 = 0.4 to 1.2 nM, determined by the concentration dependent inhibition of human MMP-2 hydrolysis of a peptide substrate （7-methoxycoumarin-4-yl）acetyl-Pro-Leu-Gly-Leu-（3-[2, 4-dinitrophenyl]-L-2, 3-diaminopropionyl）-Ala-Arg-NH2, under conditions in which recombinant pro-MMP-2 is activated, ａnd the peptide substrate concentration is 5μM. Usage ProSpec＇s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
DKK1 Human, HEK/重组人Dickkopf相关蛋白1, HEK
产品编号：PRO-815
产品规格：2μg/10μg/0.1mg
Synonyms DKK-1, DKK1, HDKK1, HDKK-1, Dickkopf-1, Dickkopf-related protein 1, SK. Introduction DKK1 is part of the dickkopf family. DKK1 is a secreted protein with 2 cysteine rich regions ａnd takes part in embryonic development through its inhibition of the WNT signaling pathway. High levels of DKK1 in bone marrow plasma ａnd peripheral blood is associated with the presence of osteolytic bone lesions in patients with multiple myeloma. Induction of DKK1 contributes to the pathological cascade triggered by beta-amyloid ａnd is significantly involved in the development of tau phosphorylation. DKK1 reduces the differentiation of osteoblastic precursor cells. DKK1 is an important member in multiple myeloma bone disease. DKK1 expression is reduced in human colon tumors, implying that DKK-1 plays a role as a tumor suppressor gene in this neoplasia; the Wnt/beta-catenin pathway is downregulated by the induction of DKK1 expression, a mechanism that is missing in colon cancer. Description DKK1 Human Recombinant produced in HEK-293 cells is a single, glycosylated, polypeptide chain containing 184 amino acids with a molecular weight of 20.7 kDa though on SDS-PAGE migrates at about 28kDa due to the glycosilation. The DKK1 is purified by proprietary chromatographic techniques. Source HEK293-F Cells. Physical Appearance Sterile Filtered White lyophilized （freeze-dried） powder. Formulation The DKK1 protein was lyophilized from a 0.2μm filtered solution containing no additives or preservatives. Solubility It is recommended to reconstitute the lyophilized DKK1 in sterile 18MΩ-cm H2O not less than 100μg/ml, which can then be further diluted to other aqueous solutions. Stability Lyophilized DKK1 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution DKK1 should be stored at 4°C between 2-7 days ａnd for future use below -18°C. For long term storage it is recommended to add a carrier protein （0.1% HSA or BSA）. Please prevent freeze-thaw cycles. Purity Greater than 95.0% as determined by SDS-PAGE. Amino acid sequence CTCVPPHPQT AFCNSDLVIR AKFVGTPEVN QTTLYQRYEI KMTKMYKGFQALGDAADIRF VYTPAMESVC GYFHRSHNRS EEFLIAGKLQ DGLLHITTCS FVAPWNSLSL AQRRGFTKTY TVGCEECTVF PCLSIPCKLQ SGTHCLWTDQ LLGSEKGFQ SRHLACLPRE PGLCTWQSLR SQIA. Biological Activity IC50 = 0.4 to 1.2 nM, determined by the concentration dependent inhibition of human MMP-2 hydrolysis of a peptide substrate （7-methoxycoumarin-4-yl）acetyl-Pro-Leu-Gly-Leu-（3-[2, 4-dinitrophenyl]-L-2, 3-diaminopropionyl）-Ala-Arg-NH2, under conditions in which recombinant pro-MMP-2 is activated, ａnd the peptide substrate concentration is 5μM. Usage ProSpec＇s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
DKK1 Human, HEK/重组人Dickkopf相关蛋白1, HEK
产品编号：PRO-815
产品规格：2μg/10μg/0.1mg
Synonyms DKK-1, DKK1, HDKK1, HDKK-1, Dickkopf-1, Dickkopf-related protein 1, SK. Introduction DKK1 is part of the dickkopf family. DKK1 is a secreted protein with 2 cysteine rich regions ａnd takes part in embryonic development through its inhibition of the WNT signaling pathway. High levels of DKK1 in bone marrow plasma ａnd peripheral blood is associated with the presence of osteolytic bone lesions in patients with multiple myeloma. Induction of DKK1 contributes to the pathological cascade triggered by beta-amyloid ａnd is significantly involved in the development of tau phosphorylation. DKK1 reduces the differentiation of osteoblastic precursor cells. DKK1 is an important member in multiple myeloma bone disease. DKK1 expression is reduced in human colon tumors, implying that DKK-1 plays a role as a tumor suppressor gene in this neoplasia; the Wnt/beta-catenin pathway is downregulated by the induction of DKK1 expression, a mechanism that is missing in colon cancer. Description DKK1 Human Recombinant produced in HEK-293 cells is a single, glycosylated, polypeptide chain containing 184 amino acids with a molecular weight of 20.7 kDa though on SDS-PAGE migrates at about 28kDa due to the glycosilation. The DKK1 is purified by proprietary chromatographic techniques. Source HEK293-F Cells. Physical Appearance Sterile Filtered White lyophilized （freeze-dried） powder. Formulation The DKK1 protein was lyophilized from a 0.2μm filtered solution containing no additives or preservatives. Solubility It is recommended to reconstitute the lyophilized DKK1 in sterile 18MΩ-cm H2O not less than 100μg/ml, which can then be further diluted to other aqueous solutions. Stability Lyophilized DKK1 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution DKK1 should be stored at 4°C between 2-7 days ａnd for future use below -18°C. For long term storage it is recommended to add a carrier protein （0.1% HSA or BSA）. Please prevent freeze-thaw cycles. Purity Greater than 95.0% as determined by SDS-PAGE. Amino acid sequence CTCVPPHPQT AFCNSDLVIR AKFVGTPEVN QTTLYQRYEI KMTKMYKGFQALGDAADIRF VYTPAMESVC GYFHRSHNRS EEFLIAGKLQ DGLLHITTCS FVAPWNSLSL AQRRGFTKTY TVGCEECTVF PCLSIPCKLQ SGTHCLWTDQ LLGSEKGFQ SRHLACLPRE PGLCTWQSLR SQIA. Biological Activity IC50 = 0.4 to 1.2 nM, determined by the concentration dependent inhibition of human MMP-2 hydrolysis of a peptide substrate （7-methoxycoumarin-4-yl）acetyl-Pro-Leu-Gly-Leu-（3-[2, 4-dinitrophenyl]-L-2, 3-diaminopropionyl）-Ala-Arg-NH2, under conditions in which recombinant pro-MMP-2 is activated, ａnd the peptide substrate concentration is 5μM. Usage ProSpec＇s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
DKK1 Human, HEK/重组人Dickkopf相关蛋白1, HEK
产品编号：PRO-815
产品规格：2μg/10μg/0.1mg
Synonyms DKK-1, DKK1, HDKK1, HDKK-1, Dickkopf-1, Dickkopf-related protein 1, SK. Introduction DKK1 is part of the dickkopf family. DKK1 is a secreted protein with 2 cysteine rich regions ａnd takes part in embryonic development through its inhibition of the WNT signaling pathway. High levels of DKK1 in bone marrow plasma ａnd peripheral blood is associated with the presence of osteolytic bone lesions in patients with multiple myeloma. Induction of DKK1 contributes to the pathological cascade triggered by beta-amyloid ａnd is significantly involved in the development of tau phosphorylation. DKK1 reduces the differentiation of osteoblastic precursor cells. DKK1 is an important member in multiple myeloma bone disease. DKK1 expression is reduced in human colon tumors, implying that DKK-1 plays a role as a tumor suppressor gene in this neoplasia; the Wnt/beta-catenin pathway is downregulated by the induction of DKK1 expression, a mechanism that is missing in colon cancer. Description DKK1 Human Recombinant produced in HEK-293 cells is a single, glycosylated, polypeptide chain containing 184 amino acids with a molecular weight of 20.7 kDa though on SDS-PAGE migrates at about 28kDa due to the glycosilation. The DKK1 is purified by proprietary chromatographic techniques. Source HEK293-F Cells. Physical Appearance Sterile Filtered White lyophilized （freeze-dried） powder. Formulation The DKK1 protein was lyophilized from a 0.2μm filtered solution containing no additives or preservatives. Solubility It is recommended to reconstitute the lyophilized DKK1 in sterile 18MΩ-cm H2O not less than 100μg/ml, which can then be further diluted to other aqueous solutions. Stability Lyophilized DKK1 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution DKK1 should be stored at 4°C between 2-7 days ａnd for future use below -18°C. For long term storage it is recommended to add a carrier protein （0.1% HSA or BSA）. Please prevent freeze-thaw cycles. Purity Greater than 95.0% as determined by SDS-PAGE. Amino acid sequence CTCVPPHPQT AFCNSDLVIR AKFVGTPEVN QTTLYQRYEI KMTKMYKGFQALGDAADIRF VYTPAMESVC GYFHRSHNRS EEFLIAGKLQ DGLLHITTCS FVAPWNSLSL AQRRGFTKTY TVGCEECTVF PCLSIPCKLQ SGTHCLWTDQ LLGSEKGFQ SRHLACLPRE PGLCTWQSLR SQIA. Biological Activity IC50 = 0.4 to 1.2 nM, determined by the concentration dependent inhibition of human MMP-2 hydrolysis of a peptide substrate （7-methoxycoumarin-4-yl）acetyl-Pro-Leu-Gly-Leu-（3-[2, 4-dinitrophenyl]-L-2, 3-diaminopropionyl）-Ala-Arg-NH2, under conditions in which recombinant pro-MMP-2 is activated, ａnd the peptide substrate concentration is 5μM. Usage ProSpec＇s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
DKK1 Human, HEK/重组人Dickkopf相关蛋白1, HEK
产品编号：PRO-815
产品规格：2μg/10μg/0.1mg
产品规格：2μg/10μg/0.1mg
| Synonyms | DKK-1, DKK1, HDKK1, HDKK-1, Dickkopf-1, Dickkopf-related protein 1, SK. |
| Introduction | DKK1 is part of the dickkopf family. DKK1 is a secreted protein with 2 cysteine rich regions ａnd takes part in embryonic development through its inhibition of the WNT signaling pathway. High levels of DKK1 in bone marrow plasma ａnd peripheral blood is associated with the presence of osteolytic bone lesions in patients with multiple myeloma. Induction of DKK1 contributes to the pathological cascade triggered by beta-amyloid ａnd is significantly involved in the development of tau phosphorylation. DKK1 reduces the differentiation of osteoblastic precursor cells. DKK1 is an important member in multiple myeloma bone disease. DKK1 expression is reduced in human colon tumors, implying that DKK-1 plays a role as a tumor suppressor gene in this neoplasia; the Wnt/beta-catenin pathway is downregulated by the induction of DKK1 expression, a mechanism that is missing in colon cancer. |
| Description | DKK1 Human Recombinant produced in HEK-293 cells is a single, glycosylated, polypeptide chain containing 184 amino acids with a molecular weight of 20.7 kDa though on SDS-PAGE migrates at about 28kDa due to the glycosilation. The DKK1 is purified by proprietary chromatographic techniques. |
| Source | HEK293-F Cells. |
| Physical Appearance | Sterile Filtered White lyophilized （freeze-dried） powder. |
| Formulation | The DKK1 protein was lyophilized from a 0.2μm filtered solution containing no additives or preservatives. |
| Solubility | It is recommended to reconstitute the lyophilized DKK1 in sterile 18MΩ-cm H2O not less than 100μg/ml, which can then be further diluted to other aqueous solutions. |
| Stability | Lyophilized DKK1 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution DKK1 should be stored at 4°C between 2-7 days ａnd for future use below -18°C. For long term storage it is recommended to add a carrier protein （0.1% HSA or BSA）. Please prevent freeze-thaw cycles. |
| Purity | Greater than 95.0% as determined by SDS-PAGE. |
| Amino acid sequence | CTCVPPHPQT AFCNSDLVIR AKFVGTPEVN QTTLYQRYEI KMTKMYKGFQALGDAADIRF VYTPAMESVC GYFHRSHNRS EEFLIAGKLQ DGLLHITTCS FVAPWNSLSL AQRRGFTKTY TVGCEECTVF PCLSIPCKLQ SGTHCLWTDQ LLGSEKGFQ SRHLACLPRE PGLCTWQSLR SQIA. |
| Biological Activity | IC50 = 0.4 to 1.2 nM, determined by the concentration dependent inhibition of human MMP-2 hydrolysis of a peptide substrate （7-methoxycoumarin-4-yl）acetyl-Pro-Leu-Gly-Leu-（3-[2, 4-dinitrophenyl]-L-2, 3-diaminopropionyl）-Ala-Arg-NH2, under conditions in which recombinant pro-MMP-2 is activated, ａnd the peptide substrate concentration is 5μM. |
| Usage | ProSpec＇s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals. |
