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慧嘉生物您实验身边的好伙伴
慧嘉生物您实验身边的好伙伴
慧嘉生物您实验身边的好伙伴
慧嘉生物您实验身边的好伙伴
慧嘉生物您实验身边的好伙伴
慧嘉生物您实验身边的好伙伴
慧嘉生物您实验身边的好伙伴
慧嘉生物您实验身边的好伙伴
慧嘉生物您实验身边的好伙伴
慧嘉生物您实验身边的好伙伴
慧嘉生物您实验身边的好伙伴
慧嘉生物您实验身边的好伙伴
慧嘉生物您实验身边的好伙伴
慧嘉生物您实验身边的好伙伴
慧嘉生物您实验身边的好伙伴
慧嘉生物您实验身边的好伙伴
慧嘉生物您实验身边的好伙伴
慧嘉生物您实验身边的好伙伴
慧嘉生物您实验身边的好伙伴
慧嘉生物您实验身边的好伙伴
慧嘉生物您实验身边的好伙伴
慧嘉生物您实验身边的好伙伴
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Vimentin Human/重组人波形蛋白
产品编号：PRO-309
产品规格：2μg/10μg/1mg
Synonyms Vimentin, Vim, FLJ36605. Introduction Vimentin expression in human malignant glioma cells depends on cellular density, algorithms of drug delivery ａnd chemo/radio treatment. Vimentin ａnd detyrosinated microtubules provide structural support for the extensive microtentacles observed in detached tumor cells ａnd a mechanism to promote successful metastatic spread. Primary colorectal carcinomas display aberrant expression of vimentin, ａnd have activated Notch ａnd TGFbeta signaling pathways. Vimentin is a strong arterial substrate for transglutaminases. Transglutaminase-mediated vimentin dimerization results in a novel unifying pathway by which vasodilatory ａnd remodeling responses may be regulated. Ablation of vimentin expression inhibits migration ａnd invasion of colon ａnd breast cancer cell lines. Vimentin is the main intermediate filament protein in mesenchymal cells ａnd is therefore of value in the differential diagnosis of undifferentiated neoplasms. Description Vimentin Human Recombinant is produced in E.Coli, having a molecular weight of 53,685 dalton （calaulated from sequence）, 57,000 dalton （determinated by SDS gelelctrophoresis）. Source Escherichia Coli. Physical Appearance Sterile Filtered White lyophilized （freeze-dried） powder. Formulation The protein （1mg/ml） was lyophilized with 30mM Tris/HCL, pH 8, 9.5M urea, 2mM EDTA, 2mM DTT 10mM methylammonium chloride. Solubility It is recommended to reconstitute the Vimentin in sterile 18MΩ-cm H2O not less than 100μg/ml, which can then be further diluted to other aqueous solutions. Stability Lyophilized Vimentin although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Vimentin should be stored at 4°C between 2-7 days ａnd for future use below -18°C.
For long term storage it is recommended to add a carrier protein （0.1% HSA or BSA）.
Please prevent freeze-thaw cycles. Reconstitution to filaments After vimentin is dissolved in 9.5M urea buffer （see above）, protofilaments ａnd filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4M urea ａnd then to low salt condition （50mM NaCl, 2mM dithiothreitol, 10mM Tris-HCl, pH 7.4）. For immunization purposes, the solution can be further dialyzed against PBS （phosphate buffered saline, e.g. Dulbecco＇s PBS）.
- Hatzfeld M. ａnd Franke W.W. （1985）. J. Cell Biol. 101, 1826-1841
- Hatzfeld M. et al. （1987）. J. Mol. Biol. 197, 237-255. Purity Greater than 95.0% as determined by SDS-gelelctrophoresis. Usage Prospec＇s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Vimentin Human/重组人波形蛋白
产品编号：PRO-309
产品规格：2μg/10μg/1mg
Synonyms Vimentin, Vim, FLJ36605. Introduction Vimentin expression in human malignant glioma cells depends on cellular density, algorithms of drug delivery ａnd chemo/radio treatment. Vimentin ａnd detyrosinated microtubules provide structural support for the extensive microtentacles observed in detached tumor cells ａnd a mechanism to promote successful metastatic spread. Primary colorectal carcinomas display aberrant expression of vimentin, ａnd have activated Notch ａnd TGFbeta signaling pathways. Vimentin is a strong arterial substrate for transglutaminases. Transglutaminase-mediated vimentin dimerization results in a novel unifying pathway by which vasodilatory ａnd remodeling responses may be regulated. Ablation of vimentin expression inhibits migration ａnd invasion of colon ａnd breast cancer cell lines. Vimentin is the main intermediate filament protein in mesenchymal cells ａnd is therefore of value in the differential diagnosis of undifferentiated neoplasms. Description Vimentin Human Recombinant is produced in E.Coli, having a molecular weight of 53,685 dalton （calaulated from sequence）, 57,000 dalton （determinated by SDS gelelctrophoresis）. Source Escherichia Coli. Physical Appearance Sterile Filtered White lyophilized （freeze-dried） powder. Formulation The protein （1mg/ml） was lyophilized with 30mM Tris/HCL, pH 8, 9.5M urea, 2mM EDTA, 2mM DTT 10mM methylammonium chloride. Solubility It is recommended to reconstitute the Vimentin in sterile 18MΩ-cm H2O not less than 100μg/ml, which can then be further diluted to other aqueous solutions. Stability Lyophilized Vimentin although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Vimentin should be stored at 4°C between 2-7 days ａnd for future use below -18°C.
For long term storage it is recommended to add a carrier protein （0.1% HSA or BSA）.
Please prevent freeze-thaw cycles. Reconstitution to filaments After vimentin is dissolved in 9.5M urea buffer （see above）, protofilaments ａnd filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4M urea ａnd then to low salt condition （50mM NaCl, 2mM dithiothreitol, 10mM Tris-HCl, pH 7.4）. For immunization purposes, the solution can be further dialyzed against PBS （phosphate buffered saline, e.g. Dulbecco＇s PBS）.
- Hatzfeld M. ａnd Franke W.W. （1985）. J. Cell Biol. 101, 1826-1841
- Hatzfeld M. et al. （1987）. J. Mol. Biol. 197, 237-255. Purity Greater than 95.0% as determined by SDS-gelelctrophoresis. Usage Prospec＇s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Vimentin Human/重组人波形蛋白
产品编号：PRO-309
产品规格：2μg/10μg/1mg
Synonyms Vimentin, Vim, FLJ36605. Introduction Vimentin expression in human malignant glioma cells depends on cellular density, algorithms of drug delivery ａnd chemo/radio treatment. Vimentin ａnd detyrosinated microtubules provide structural support for the extensive microtentacles observed in detached tumor cells ａnd a mechanism to promote successful metastatic spread. Primary colorectal carcinomas display aberrant expression of vimentin, ａnd have activated Notch ａnd TGFbeta signaling pathways. Vimentin is a strong arterial substrate for transglutaminases. Transglutaminase-mediated vimentin dimerization results in a novel unifying pathway by which vasodilatory ａnd remodeling responses may be regulated. Ablation of vimentin expression inhibits migration ａnd invasion of colon ａnd breast cancer cell lines. Vimentin is the main intermediate filament protein in mesenchymal cells ａnd is therefore of value in the differential diagnosis of undifferentiated neoplasms. Description Vimentin Human Recombinant is produced in E.Coli, having a molecular weight of 53,685 dalton （calaulated from sequence）, 57,000 dalton （determinated by SDS gelelctrophoresis）. Source Escherichia Coli. Physical Appearance Sterile Filtered White lyophilized （freeze-dried） powder. Formulation The protein （1mg/ml） was lyophilized with 30mM Tris/HCL, pH 8, 9.5M urea, 2mM EDTA, 2mM DTT 10mM methylammonium chloride. Solubility It is recommended to reconstitute the Vimentin in sterile 18MΩ-cm H2O not less than 100μg/ml, which can then be further diluted to other aqueous solutions. Stability Lyophilized Vimentin although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Vimentin should be stored at 4°C between 2-7 days ａnd for future use below -18°C.
For long term storage it is recommended to add a carrier protein （0.1% HSA or BSA）.
Please prevent freeze-thaw cycles. Reconstitution to filaments After vimentin is dissolved in 9.5M urea buffer （see above）, protofilaments ａnd filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4M urea ａnd then to low salt condition （50mM NaCl, 2mM dithiothreitol, 10mM Tris-HCl, pH 7.4）. For immunization purposes, the solution can be further dialyzed against PBS （phosphate buffered saline, e.g. Dulbecco＇s PBS）.
- Hatzfeld M. ａnd Franke W.W. （1985）. J. Cell Biol. 101, 1826-1841
- Hatzfeld M. et al. （1987）. J. Mol. Biol. 197, 237-255. Purity Greater than 95.0% as determined by SDS-gelelctrophoresis. Usage Prospec＇s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Vimentin Human/重组人波形蛋白
产品编号：PRO-309
产品规格：2μg/10μg/1mg
Synonyms Vimentin, Vim, FLJ36605. Introduction Vimentin expression in human malignant glioma cells depends on cellular density, algorithms of drug delivery ａnd chemo/radio treatment. Vimentin ａnd detyrosinated microtubules provide structural support for the extensive microtentacles observed in detached tumor cells ａnd a mechanism to promote successful metastatic spread. Primary colorectal carcinomas display aberrant expression of vimentin, ａnd have activated Notch ａnd TGFbeta signaling pathways. Vimentin is a strong arterial substrate for transglutaminases. Transglutaminase-mediated vimentin dimerization results in a novel unifying pathway by which vasodilatory ａnd remodeling responses may be regulated. Ablation of vimentin expression inhibits migration ａnd invasion of colon ａnd breast cancer cell lines. Vimentin is the main intermediate filament protein in mesenchymal cells ａnd is therefore of value in the differential diagnosis of undifferentiated neoplasms. Description Vimentin Human Recombinant is produced in E.Coli, having a molecular weight of 53,685 dalton （calaulated from sequence）, 57,000 dalton （determinated by SDS gelelctrophoresis）. Source Escherichia Coli. Physical Appearance Sterile Filtered White lyophilized （freeze-dried） powder. Formulation The protein （1mg/ml） was lyophilized with 30mM Tris/HCL, pH 8, 9.5M urea, 2mM EDTA, 2mM DTT 10mM methylammonium chloride. Solubility It is recommended to reconstitute the Vimentin in sterile 18MΩ-cm H2O not less than 100μg/ml, which can then be further diluted to other aqueous solutions. Stability Lyophilized Vimentin although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Vimentin should be stored at 4°C between 2-7 days ａnd for future use below -18°C.
For long term storage it is recommended to add a carrier protein （0.1% HSA or BSA）.
Please prevent freeze-thaw cycles. Reconstitution to filaments After vimentin is dissolved in 9.5M urea buffer （see above）, protofilaments ａnd filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4M urea ａnd then to low salt condition （50mM NaCl, 2mM dithiothreitol, 10mM Tris-HCl, pH 7.4）. For immunization purposes, the solution can be further dialyzed against PBS （phosphate buffered saline, e.g. Dulbecco＇s PBS）.
- Hatzfeld M. ａnd Franke W.W. （1985）. J. Cell Biol. 101, 1826-1841
- Hatzfeld M. et al. （1987）. J. Mol. Biol. 197, 237-255. Purity Greater than 95.0% as determined by SDS-gelelctrophoresis. Usage Prospec＇s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Vimentin Human/重组人波形蛋白
产品编号：PRO-309
产品规格：2μg/10μg/1mg
Synonyms Vimentin, Vim, FLJ36605. Introduction Vimentin expression in human malignant glioma cells depends on cellular density, algorithms of drug delivery ａnd chemo/radio treatment. Vimentin ａnd detyrosinated microtubules provide structural support for the extensive microtentacles observed in detached tumor cells ａnd a mechanism to promote successful metastatic spread. Primary colorectal carcinomas display aberrant expression of vimentin, ａnd have activated Notch ａnd TGFbeta signaling pathways. Vimentin is a strong arterial substrate for transglutaminases. Transglutaminase-mediated vimentin dimerization results in a novel unifying pathway by which vasodilatory ａnd remodeling responses may be regulated. Ablation of vimentin expression inhibits migration ａnd invasion of colon ａnd breast cancer cell lines. Vimentin is the main intermediate filament protein in mesenchymal cells ａnd is therefore of value in the differential diagnosis of undifferentiated neoplasms. Description Vimentin Human Recombinant is produced in E.Coli, having a molecular weight of 53,685 dalton （calaulated from sequence）, 57,000 dalton （determinated by SDS gelelctrophoresis）. Source Escherichia Coli. Physical Appearance Sterile Filtered White lyophilized （freeze-dried） powder. Formulation The protein （1mg/ml） was lyophilized with 30mM Tris/HCL, pH 8, 9.5M urea, 2mM EDTA, 2mM DTT 10mM methylammonium chloride. Solubility It is recommended to reconstitute the Vimentin in sterile 18MΩ-cm H2O not less than 100μg/ml, which can then be further diluted to other aqueous solutions. Stability Lyophilized Vimentin although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Vimentin should be stored at 4°C between 2-7 days ａnd for future use below -18°C.
For long term storage it is recommended to add a carrier protein （0.1% HSA or BSA）.
Please prevent freeze-thaw cycles. Reconstitution to filaments After vimentin is dissolved in 9.5M urea buffer （see above）, protofilaments ａnd filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4M urea ａnd then to low salt condition （50mM NaCl, 2mM dithiothreitol, 10mM Tris-HCl, pH 7.4）. For immunization purposes, the solution can be further dialyzed against PBS （phosphate buffered saline, e.g. Dulbecco＇s PBS）.
- Hatzfeld M. ａnd Franke W.W. （1985）. J. Cell Biol. 101, 1826-1841
- Hatzfeld M. et al. （1987）. J. Mol. Biol. 197, 237-255. Purity Greater than 95.0% as determined by SDS-gelelctrophoresis. Usage Prospec＇s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Vimentin Human/重组人波形蛋白
产品编号：PRO-309
产品规格：2μg/10μg/1mg
Synonyms Vimentin, Vim, FLJ36605. Introduction Vimentin expression in human malignant glioma cells depends on cellular density, algorithms of drug delivery ａnd chemo/radio treatment. Vimentin ａnd detyrosinated microtubules provide structural support for the extensive microtentacles observed in detached tumor cells ａnd a mechanism to promote successful metastatic spread. Primary colorectal carcinomas display aberrant expression of vimentin, ａnd have activated Notch ａnd TGFbeta signaling pathways. Vimentin is a strong arterial substrate for transglutaminases. Transglutaminase-mediated vimentin dimerization results in a novel unifying pathway by which vasodilatory ａnd remodeling responses may be regulated. Ablation of vimentin expression inhibits migration ａnd invasion of colon ａnd breast cancer cell lines. Vimentin is the main intermediate filament protein in mesenchymal cells ａnd is therefore of value in the differential diagnosis of undifferentiated neoplasms. Description Vimentin Human Recombinant is produced in E.Coli, having a molecular weight of 53,685 dalton （calaulated from sequence）, 57,000 dalton （determinated by SDS gelelctrophoresis）. Source Escherichia Coli. Physical Appearance Sterile Filtered White lyophilized （freeze-dried） powder. Formulation The protein （1mg/ml） was lyophilized with 30mM Tris/HCL, pH 8, 9.5M urea, 2mM EDTA, 2mM DTT 10mM methylammonium chloride. Solubility It is recommended to reconstitute the Vimentin in sterile 18MΩ-cm H2O not less than 100μg/ml, which can then be further diluted to other aqueous solutions. Stability Lyophilized Vimentin although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Vimentin should be stored at 4°C between 2-7 days ａnd for future use below -18°C.
For long term storage it is recommended to add a carrier protein （0.1% HSA or BSA）.
Please prevent freeze-thaw cycles. Reconstitution to filaments After vimentin is dissolved in 9.5M urea buffer （see above）, protofilaments ａnd filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4M urea ａnd then to low salt condition （50mM NaCl, 2mM dithiothreitol, 10mM Tris-HCl, pH 7.4）. For immunization purposes, the solution can be further dialyzed against PBS （phosphate buffered saline, e.g. Dulbecco＇s PBS）.
- Hatzfeld M. ａnd Franke W.W. （1985）. J. Cell Biol. 101, 1826-1841
- Hatzfeld M. et al. （1987）. J. Mol. Biol. 197, 237-255. Purity Greater than 95.0% as determined by SDS-gelelctrophoresis. Usage Prospec＇s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Vimentin Human/重组人波形蛋白
产品编号：PRO-309
产品规格：2μg/10μg/1mg
Synonyms Vimentin, Vim, FLJ36605. Introduction Vimentin expression in human malignant glioma cells depends on cellular density, algorithms of drug delivery ａnd chemo/radio treatment. Vimentin ａnd detyrosinated microtubules provide structural support for the extensive microtentacles observed in detached tumor cells ａnd a mechanism to promote successful metastatic spread. Primary colorectal carcinomas display aberrant expression of vimentin, ａnd have activated Notch ａnd TGFbeta signaling pathways. Vimentin is a strong arterial substrate for transglutaminases. Transglutaminase-mediated vimentin dimerization results in a novel unifying pathway by which vasodilatory ａnd remodeling responses may be regulated. Ablation of vimentin expression inhibits migration ａnd invasion of colon ａnd breast cancer cell lines. Vimentin is the main intermediate filament protein in mesenchymal cells ａnd is therefore of value in the differential diagnosis of undifferentiated neoplasms. Description Vimentin Human Recombinant is produced in E.Coli, having a molecular weight of 53,685 dalton （calaulated from sequence）, 57,000 dalton （determinated by SDS gelelctrophoresis）. Source Escherichia Coli. Physical Appearance Sterile Filtered White lyophilized （freeze-dried） powder. Formulation The protein （1mg/ml） was lyophilized with 30mM Tris/HCL, pH 8, 9.5M urea, 2mM EDTA, 2mM DTT 10mM methylammonium chloride. Solubility It is recommended to reconstitute the Vimentin in sterile 18MΩ-cm H2O not less than 100μg/ml, which can then be further diluted to other aqueous solutions. Stability Lyophilized Vimentin although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Vimentin should be stored at 4°C between 2-7 days ａnd for future use below -18°C.
For long term storage it is recommended to add a carrier protein （0.1% HSA or BSA）.
Please prevent freeze-thaw cycles. Reconstitution to filaments After vimentin is dissolved in 9.5M urea buffer （see above）, protofilaments ａnd filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4M urea ａnd then to low salt condition （50mM NaCl, 2mM dithiothreitol, 10mM Tris-HCl, pH 7.4）. For immunization purposes, the solution can be further dialyzed against PBS （phosphate buffered saline, e.g. Dulbecco＇s PBS）.
- Hatzfeld M. ａnd Franke W.W. （1985）. J. Cell Biol. 101, 1826-1841
- Hatzfeld M. et al. （1987）. J. Mol. Biol. 197, 237-255. Purity Greater than 95.0% as determined by SDS-gelelctrophoresis. Usage Prospec＇s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Vimentin Human/重组人波形蛋白
产品编号：PRO-309
产品规格：2μg/10μg/1mg
Synonyms Vimentin, Vim, FLJ36605. Introduction Vimentin expression in human malignant glioma cells depends on cellular density, algorithms of drug delivery ａnd chemo/radio treatment. Vimentin ａnd detyrosinated microtubules provide structural support for the extensive microtentacles observed in detached tumor cells ａnd a mechanism to promote successful metastatic spread. Primary colorectal carcinomas display aberrant expression of vimentin, ａnd have activated Notch ａnd TGFbeta signaling pathways. Vimentin is a strong arterial substrate for transglutaminases. Transglutaminase-mediated vimentin dimerization results in a novel unifying pathway by which vasodilatory ａnd remodeling responses may be regulated. Ablation of vimentin expression inhibits migration ａnd invasion of colon ａnd breast cancer cell lines. Vimentin is the main intermediate filament protein in mesenchymal cells ａnd is therefore of value in the differential diagnosis of undifferentiated neoplasms. Description Vimentin Human Recombinant is produced in E.Coli, having a molecular weight of 53,685 dalton （calaulated from sequence）, 57,000 dalton （determinated by SDS gelelctrophoresis）. Source Escherichia Coli. Physical Appearance Sterile Filtered White lyophilized （freeze-dried） powder. Formulation The protein （1mg/ml） was lyophilized with 30mM Tris/HCL, pH 8, 9.5M urea, 2mM EDTA, 2mM DTT 10mM methylammonium chloride. Solubility It is recommended to reconstitute the Vimentin in sterile 18MΩ-cm H2O not less than 100μg/ml, which can then be further diluted to other aqueous solutions. Stability Lyophilized Vimentin although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Vimentin should be stored at 4°C between 2-7 days ａnd for future use below -18°C.
For long term storage it is recommended to add a carrier protein （0.1% HSA or BSA）.
Please prevent freeze-thaw cycles. Reconstitution to filaments After vimentin is dissolved in 9.5M urea buffer （see above）, protofilaments ａnd filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4M urea ａnd then to low salt condition （50mM NaCl, 2mM dithiothreitol, 10mM Tris-HCl, pH 7.4）. For immunization purposes, the solution can be further dialyzed against PBS （phosphate buffered saline, e.g. Dulbecco＇s PBS）.
- Hatzfeld M. ａnd Franke W.W. （1985）. J. Cell Biol. 101, 1826-1841
- Hatzfeld M. et al. （1987）. J. Mol. Biol. 197, 237-255. Purity Greater than 95.0% as determined by SDS-gelelctrophoresis. Usage Prospec＇s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Vimentin Human/重组人波形蛋白
产品编号：PRO-309
产品规格：2μg/10μg/1mg
Synonyms Vimentin, Vim, FLJ36605. Introduction Vimentin expression in human malignant glioma cells depends on cellular density, algorithms of drug delivery ａnd chemo/radio treatment. Vimentin ａnd detyrosinated microtubules provide structural support for the extensive microtentacles observed in detached tumor cells ａnd a mechanism to promote successful metastatic spread. Primary colorectal carcinomas display aberrant expression of vimentin, ａnd have activated Notch ａnd TGFbeta signaling pathways. Vimentin is a strong arterial substrate for transglutaminases. Transglutaminase-mediated vimentin dimerization results in a novel unifying pathway by which vasodilatory ａnd remodeling responses may be regulated. Ablation of vimentin expression inhibits migration ａnd invasion of colon ａnd breast cancer cell lines. Vimentin is the main intermediate filament protein in mesenchymal cells ａnd is therefore of value in the differential diagnosis of undifferentiated neoplasms. Description Vimentin Human Recombinant is produced in E.Coli, having a molecular weight of 53,685 dalton （calaulated from sequence）, 57,000 dalton （determinated by SDS gelelctrophoresis）. Source Escherichia Coli. Physical Appearance Sterile Filtered White lyophilized （freeze-dried） powder. Formulation The protein （1mg/ml） was lyophilized with 30mM Tris/HCL, pH 8, 9.5M urea, 2mM EDTA, 2mM DTT 10mM methylammonium chloride. Solubility It is recommended to reconstitute the Vimentin in sterile 18MΩ-cm H2O not less than 100μg/ml, which can then be further diluted to other aqueous solutions. Stability Lyophilized Vimentin although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Vimentin should be stored at 4°C between 2-7 days ａnd for future use below -18°C.
For long term storage it is recommended to add a carrier protein （0.1% HSA or BSA）.
Please prevent freeze-thaw cycles. Reconstitution to filaments After vimentin is dissolved in 9.5M urea buffer （see above）, protofilaments ａnd filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4M urea ａnd then to low salt condition （50mM NaCl, 2mM dithiothreitol, 10mM Tris-HCl, pH 7.4）. For immunization purposes, the solution can be further dialyzed against PBS （phosphate buffered saline, e.g. Dulbecco＇s PBS）.
- Hatzfeld M. ａnd Franke W.W. （1985）. J. Cell Biol. 101, 1826-1841
- Hatzfeld M. et al. （1987）. J. Mol. Biol. 197, 237-255. Purity Greater than 95.0% as determined by SDS-gelelctrophoresis. Usage Prospec＇s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Vimentin Human/重组人波形蛋白
产品编号：PRO-309
产品规格：2μg/10μg/1mg
Synonyms Vimentin, Vim, FLJ36605. Introduction Vimentin expression in human malignant glioma cells depends on cellular density, algorithms of drug delivery ａnd chemo/radio treatment. Vimentin ａnd detyrosinated microtubules provide structural support for the extensive microtentacles observed in detached tumor cells ａnd a mechanism to promote successful metastatic spread. Primary colorectal carcinomas display aberrant expression of vimentin, ａnd have activated Notch ａnd TGFbeta signaling pathways. Vimentin is a strong arterial substrate for transglutaminases. Transglutaminase-mediated vimentin dimerization results in a novel unifying pathway by which vasodilatory ａnd remodeling responses may be regulated. Ablation of vimentin expression inhibits migration ａnd invasion of colon ａnd breast cancer cell lines. Vimentin is the main intermediate filament protein in mesenchymal cells ａnd is therefore of value in the differential diagnosis of undifferentiated neoplasms. Description Vimentin Human Recombinant is produced in E.Coli, having a molecular weight of 53,685 dalton （calaulated from sequence）, 57,000 dalton （determinated by SDS gelelctrophoresis）. Source Escherichia Coli. Physical Appearance Sterile Filtered White lyophilized （freeze-dried） powder. Formulation The protein （1mg/ml） was lyophilized with 30mM Tris/HCL, pH 8, 9.5M urea, 2mM EDTA, 2mM DTT 10mM methylammonium chloride. Solubility It is recommended to reconstitute the Vimentin in sterile 18MΩ-cm H2O not less than 100μg/ml, which can then be further diluted to other aqueous solutions. Stability Lyophilized Vimentin although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Vimentin should be stored at 4°C between 2-7 days ａnd for future use below -18°C.
For long term storage it is recommended to add a carrier protein （0.1% HSA or BSA）.
Please prevent freeze-thaw cycles. Reconstitution to filaments After vimentin is dissolved in 9.5M urea buffer （see above）, protofilaments ａnd filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4M urea ａnd then to low salt condition （50mM NaCl, 2mM dithiothreitol, 10mM Tris-HCl, pH 7.4）. For immunization purposes, the solution can be further dialyzed against PBS （phosphate buffered saline, e.g. Dulbecco＇s PBS）.
- Hatzfeld M. ａnd Franke W.W. （1985）. J. Cell Biol. 101, 1826-1841
- Hatzfeld M. et al. （1987）. J. Mol. Biol. 197, 237-255. Purity Greater than 95.0% as determined by SDS-gelelctrophoresis. Usage Prospec＇s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
产品规格：2μg/10μg/1mg
| Synonyms | Vimentin, Vim, FLJ36605. |
| Introduction | Vimentin expression in human malignant glioma cells depends on cellular density, algorithms of drug delivery ａnd chemo/radio treatment. Vimentin ａnd detyrosinated microtubules provide structural support for the extensive microtentacles observed in detached tumor cells ａnd a mechanism to promote successful metastatic spread. Primary colorectal carcinomas display aberrant expression of vimentin, ａnd have activated Notch ａnd TGFbeta signaling pathways. Vimentin is a strong arterial substrate for transglutaminases. Transglutaminase-mediated vimentin dimerization results in a novel unifying pathway by which vasodilatory ａnd remodeling responses may be regulated. Ablation of vimentin expression inhibits migration ａnd invasion of colon ａnd breast cancer cell lines. Vimentin is the main intermediate filament protein in mesenchymal cells ａnd is therefore of value in the differential diagnosis of undifferentiated neoplasms. |
| Description | Vimentin Human Recombinant is produced in E.Coli, having a molecular weight of 53,685 dalton （calaulated from sequence）, 57,000 dalton （determinated by SDS gelelctrophoresis）. |
| Source | Escherichia Coli. |
| Physical Appearance | Sterile Filtered White lyophilized （freeze-dried） powder. |
| Formulation | The protein （1mg/ml） was lyophilized with 30mM Tris/HCL, pH 8, 9.5M urea, 2mM EDTA, 2mM DTT 10mM methylammonium chloride. |
| Solubility | It is recommended to reconstitute the Vimentin in sterile 18MΩ-cm H2O not less than 100μg/ml, which can then be further diluted to other aqueous solutions. |
| Stability | Lyophilized Vimentin although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Vimentin should be stored at 4°C between 2-7 days ａnd for future use below -18°C. For long term storage it is recommended to add a carrier protein （0.1% HSA or BSA）. Please prevent freeze-thaw cycles. |
| Reconstitution to filaments | After vimentin is dissolved in 9.5M urea buffer （see above）, protofilaments ａnd filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4M urea ａnd then to low salt condition （50mM NaCl, 2mM dithiothreitol, 10mM Tris-HCl, pH 7.4）. For immunization purposes, the solution can be further dialyzed against PBS （phosphate buffered saline, e.g. Dulbecco＇s PBS）. - Hatzfeld M. ａnd Franke W.W. （1985）. J. Cell Biol. 101, 1826-1841 - Hatzfeld M. et al. （1987）. J. Mol. Biol. 197, 237-255. |
| Purity | Greater than 95.0% as determined by SDS-gelelctrophoresis. |
| Usage | Prospec＇s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals. |
