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PCNA Human/重组人增殖细胞核抗原

点击次数:164发布时间:2012/10/30 20:27:40

PCNA Human/重组人增殖细胞核抗原

更新日期:2024/10/16 16:00:43

所 在 地:美洲

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简单介绍:PCNA Human/重组人增殖细胞核抗原

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PCNA Human/重组人增殖细胞核抗原

产品编号:PRO-615
产品规格:5μg/25μg/1mg
 

Synonyms Proliferating cell nuclear antigen, PCNA, Cyclin, MGC8367.
Introduction PCNA is located in the nucleus and is a cofactor of DNA polymerase delta. PCNA acts as a homotrimer and helps elevate the processivity of leading strand synthesis during DNA replication. In reaction to DNA damage, PCNA is ubiquitinated and takes part in the RAD6-dependent DNA repair pathway. 2 transcript variants encoding the same protein have been found for PCNA gene. Pseudogenes of this PCNA gene have been described on chromosome 4 and on the X chromosome. PCNA is expressed during late G1- phase, S-phase of mitosis and persists until the end of the M-phase because of its long biological half-life. PCNA is induced by UV irradiation.
Description PCNA Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 261 amino acids and having a molecular mass of 28769 Dalton.
Source Escherichia Coli.
Physical Appearance Sterile Filtered colorless solution.
Formulation The PCNA solution contains 20mM Tris pH-7.5 & 2mM EDTA & 20% glycerol.
Stability PCNA although stable 4°C for 4 weeks, should be stored desiccated below -18°C.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.
Amino Acid Sequence MFEARLVQGS ILKKVLEALK DLINEACWDI SSSGVNLQSM DSSHVSLVQLTLRSEGFDTY RCDRNLAMGV NLTSMSKILK CAGNEDIITL RAEDNADTLA LVFEAPNQEK VSDYEMKLMD LDVEQLGIPE QEYSCVVKMP SGEFARICRD LSHIGDAVVI SCAKDGVKFS ASGELGNGNI KLSQTSNVDK EEEAVTIEMN EPVQLTFALR YLNFFTKATP LSSTVTLSMS ADVPLVVEYK IADMGHLKYY LAPKIEDEEG S.
Purity Greater than 95.0% as determined by(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Usage ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

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